Dentin induction by implants of autolyzed antigen-extracted allogeneic dentin on amputated pulps of dogs

Implantation of autolyzed antigen-extracted allogeneic (AAA) dentin matrix gelatin powder caused homeoinduction on amputated dental pulps. This event began with migration of spindle-shaped mesenchymal cells into the cavity on the amputated pulp. This was followed by proliferation of undifferentiated large cells concomitantly with vascular invasion and attachment of spindle-shaped cells and large cells to the AAA dentin. The adhering cells differentiated into osteodentinoblasts and/or preodontoblasts to form osteodentin matrix and predentin respectively. Osteodentinocytes and odontoblasts, then, formed osteodentin and tubular dentin. The healing of pulp dressed with inactivated AAA dentin using guanidine HCl was delayed. These results suggested that AAA dentin matrix powder may have chemotactic and mitogenic activity for undifferentiated mesenchymal cells and may provide a suitable scaffolding for fixation of these cells. Compartments of microenvironment formed by AAA dentin and the enveloping hard substances may play some role in differentiation into odontoblasts.