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Updated: Jan 18, 2026

Rapid Assessment of Membrane Protein Quality by Fluorescent Size Exclusion Chromatography
Published on: January 6, 2023
Robust and convenient analysis of protein thermal and chemical stability
Markus Niklasson1, Cecilia Andresen1, Sara Helander2
1Division of Chemistry, Department of Physics Chemistry and Biology, Linköping University, 581 83 Linköping, Sweden.
Abstract:
We present the software CDpal that is used to analyze thermal and chemical denaturation data to obtain information on protein stability. The software uses standard assumptions and equations applied to two-state and various types of three-state denaturation models in order to determine thermodynamic parameters. It can analyze denaturation monitored by both circular dichroism and fluorescence spectroscopy and is extremely flexible in terms of input format. Furthermore, it is intuitive and easy to use because of the graphical user interface and extensive documentation. As illustrated by the examples herein, CDpal should be a valuable tool for analysis of protein stability.
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