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UT-B Urea Transporter Localization in the Bovine Gastrointestinal Tract
J Coyle1, S McDaid1, C Walpole1
1Room 2.55, School of Biology and Environmental Science, University College Dublin Science Centre West, Dublin 4, Ireland.
The Journal of Membrane Biology
|September 26, 2015
Summary
Facilitative UT-B urea transporters are crucial for urea nitrogen salvaging in ruminants. This study reveals UT-B2 is the primary urea transporter in bovine ruminal sacs, localized to the plasma membrane of specific cells.
Area of Science:
- Physiology
- Molecular Biology
- Animal Science
Background:
- Facilitative UT-B urea transporters are vital for urea nitrogen salvaging in the gastrointestinal tract of mammals, especially ruminants.
- Previous studies identified UT-B transporters in cows, sheep, and goats.
Purpose of the Study:
- To investigate the localization of UT-B transporters throughout the bovine gastrointestinal tract.
- To identify the predominant UT-B transcript and protein in different regions of the bovine gut.
Main Methods:
- RT-PCR to analyze UT-B mRNA expression.
- Immunoblotting to detect UT-B protein presence and size.
- Immunolocalization to determine the cellular and subcellular location of UT-B proteins.
Main Results:
- UT-B2 was the predominant UT-B mRNA transcript in all three ruminal sacs (dorsal, ventral, cranial).
- A ~50 kDa glycosylated UT-B2 protein was detected in all ruminal sacs.
- UT-B2 protein was localized to the plasma membrane of stratum basale cells in ruminal papillae.
- Other UT-B proteins were found in the basolateral membranes of epithelial cells in the abomasum, colon, and rectum.
Conclusions:
- UT-B2 exhibits consistent cellular localization across all bovine ruminal sacs.
- Distinct UT-B proteins are present in epithelial cells of various bovine gastrointestinal tissues, playing roles in urea transport.
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