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Genome-wide Mapping of Protein-DNA Interactions with ChEC-seq in Saccharomyces cerevisiae
Published on: June 3, 2017
Chromatin Endogenous Cleavage (ChEC) as a Method to Quantify Protein Interaction with Genomic DNA in Saccharomyces
Virginia Babl1, Ulrike Stöckl1, Herbert Tschochner2
1Lehrstuhl Biochemie III, Biochemie-Zentrum Regensburg (BZR), Universität Regensburg, Universitaetsstr. 31, 93053, Regensburg, Germany.
Abstract:
Chromatin endogenous cleavage (ChEC) is a technique which allows to monitor protein-DNA interaction in the nucleus of eukaryotic cells. In addition to mapping of genomic interaction sites ChEC may also yield quantitative information about the occupancy of proteins at their genomic target regions. Here, we provide a protocol for ChEC experiments in S. cerevisiae, downstream DNA analysis and quantification of ChEC-mediated degradation. The potential of the method is exemplified in ChEC experiments with RNA polymerase I and the yeast homolog of linker histone H1.
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