Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Apr 3, 2026

Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function
07:34

Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function

Published on: May 5, 2023

5.3K

Reference Genes in the Pathosystem Phakopsora pachyrhizi/ Soybean Suitable for Normalization in Transcript Profiling.

Daniela Hirschburger1, Manuel Müller2, Ralf T Voegele3

  • 1Department of Phytopathology, Institute of Phytomedicine, Faculty of Agricultural Sciences, University of Hohenheim, Otto-Sander-Straße 5, 70599 Stuttgart, Germany. Daniela.Hirschburger@uni-hohenheim.de.

International Journal of Molecular Sciences
|September 26, 2015
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mutations in the complex III of Zymoseptoria tritici and implications on fungicide sensitivity and fitness.

Pesticide biochemistry and physiology·2025
Same author

Modification of inhibitory control and craving through transcranial direct current stimulation as an add-on treatment for substance use disorder: protocol for a randomized controlled study.

BMC psychology·2025
Same author

Investigation of the combined effects of cannabidiol plus naltrexone on alcohol craving in alcohol dependence: study protocol of a phase II randomised, double-blind, placebo-controlled, parallel-group trial - ICONICplus Trial.

BMJ open·2025
Same author

Glucose Intake Reduces Craving in Patients with Alcohol Use Disorder Depending on Insulin Response.

European addiction research·2025
Same author

Analysis of the genetic diversity of the soybean rust pathogen Phakopsora pachyrhizi reveals two major evolutionary lineages.

Fungal genetics and biology : FG & B·2025
Same author

Glucose intake reduces alcohol craving and amplifies habituation to cue-induced brain activation in male patients with alcohol use disorder: A randomized, placebo-controlled crossover study examining male and female patients with AUD.

Psychoneuroendocrinology·2025
This summary is machine-generated.

Accurate soybean rust research requires stable reference genes for gene expression analysis. This study identifies optimal reference genes for Phakopsora pachyrhizi and Glycine max during infection, crucial for developing Host Induced Gene Silencing strategies.

Area of Science:

  • Plant pathology
  • Molecular biology
  • Biotechnology

Background:

  • Soybean rust, caused by Phakopsora pachyrhizi, poses a significant threat to global soybean production.
  • Host Induced Gene Silencing (HIGS) and effector protein studies offer promising avenues for pathogen control.
  • Accurate quantification of transcript abundance via RT-qPCR is essential for these approaches.

Purpose of the Study:

  • To identify and validate stable reference genes for normalizing RT-qPCR data in P. pachyrhizi and Glycine max during the soybean rust infection process.
  • To ensure reliable gene expression analysis in accordance with MIQE guidelines.

Main Methods:

  • Candidate reference genes from both P. pachyrhizi and G. max were evaluated.
  • Stability analysis was performed using geNorm and NormFinder algorithms.
Keywords:
HIGS/VIGSPhakopsora pachyrhiziRT-qPCReffectorreference gene

More Related Videos

Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform
09:13

Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform

Published on: January 13, 2016

8.4K
Maintaining Biological Cultures and Measuring Gene Expression in Aphis nerii: A Non-model System for Plant-insect Interactions
07:20

Maintaining Biological Cultures and Measuring Gene Expression in Aphis nerii: A Non-model System for Plant-insect Interactions

Published on: August 31, 2018

8.1K

Related Experiment Videos

Last Updated: Apr 3, 2026

Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function
07:34

Author Spotlight: Soybean Hairy Root Transformation for the Analysis of Gene Function

Published on: May 5, 2023

5.3K
Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform
09:13

Gene Expression Profiling of Infecting Microbes Using a Digital Bar-coding Platform

Published on: January 13, 2016

8.4K
Maintaining Biological Cultures and Measuring Gene Expression in Aphis nerii: A Non-model System for Plant-insect Interactions
07:20

Maintaining Biological Cultures and Measuring Gene Expression in Aphis nerii: A Non-model System for Plant-insect Interactions

Published on: August 31, 2018

8.1K
  • RT-qPCR was employed to quantify transcript abundance across different infection stages.

    • Main Results:

    • For in planta P. pachyrhizi experiments, CytB and PDK or GAPDH are recommended.
    • For in vitro stages and the entire infection process, RPS14 and UbcE2 (or CytB) are suitable.
    • For soybean genes during rust infection, Ukn2 and cons7 are recommended.

    Conclusions:

    • Validated reference genes are critical for accurate gene expression studies in soybean rust research.
    • Specific gene combinations are recommended for different experimental conditions and stages.
    • This research supports the advancement of HIGS and effector studies for soybean rust management.