Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulated Protein Degradation02:58

Regulated Protein Degradation

9.3K
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
9.3K
Regulated Protein Degradation02:58

Regulated Protein Degradation

3.4K
3.4K
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

9.9K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
9.9K
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

3.5K
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
3.5K
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

1.8K
1.8K
The Proteasome01:13

The Proteasome

2.0K
Eukaryotic cells can degrade proteins through several pathways. One of the most important among these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
2.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multi-Property Optimization of Antimicrobial Peptides Using Reinforcement Learning and Conditional Independence Regularization.

IEEE journal of biomedical and health informatics·2026
Same author

Cryo-EM analysis of cooperative conformational changes in the SARS-CoV-2 spike protein trimer.

IUCrJ·2026
Same author

Mitigating Electrode Stress via Self-Constructed Interfacial Carrier Networks in High-Areal-Capacity SiO<sub><i>x</i></sub> Anodes.

Journal of the American Chemical Society·2026
Same author

Comparative reproductive toxicity of 6:2 FTS and PFOS in female zebrafish (Danio rerio): From histopathology to molecular response.

Ecotoxicology and environmental safety·2026
Same author

A chromosome-level assembly of the alpine snow alga Chloromonas typhlos.

Scientific data·2026
Same author

Long-term B-cell depletion with rituximab in relapsing, refractory and severe lupus nephritis: a retrospective case series.

Clinical kidney journal·2026

Related Experiment Video

Updated: Apr 3, 2026

In-vitro Reconstitution of Bacterial Ubiquitination and VCP/p97-mediated Elimination
07:58

In-vitro Reconstitution of Bacterial Ubiquitination and VCP/p97-mediated Elimination

Published on: January 2, 2026

622

Ciliary/Flagellar Protein Ubiquitination.

Huan Long1, Qiyu Wang2, Kaiyao Huang3

  • 1Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China. huanlong@ihb.ac.cn.

Cells
|September 26, 2015
PubMed
Summary
This summary is machine-generated.

Ubiquitination, a key post-translational modification, is crucial for ciliary and flagellar functions including assembly, motility, and signal transduction. Further research is needed to fully understand its complex roles in these essential eukaryotic organelles.

Keywords:
flagellapost-translational modificationsprimary ciliaspermubiquitination

More Related Videos

Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A EYFP-CENP-A
09:02

Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A EYFP-CENP-A

Published on: June 10, 2020

6.1K
In Vitro Analysis of E3 Ubiquitin Ligase Function
06:06

In Vitro Analysis of E3 Ubiquitin Ligase Function

Published on: May 14, 2021

6.2K

Related Experiment Videos

Last Updated: Apr 3, 2026

In-vitro Reconstitution of Bacterial Ubiquitination and VCP/p97-mediated Elimination
07:58

In-vitro Reconstitution of Bacterial Ubiquitination and VCP/p97-mediated Elimination

Published on: January 2, 2026

622
Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A EYFP-CENP-A
09:02

Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A EYFP-CENP-A

Published on: June 10, 2020

6.1K
In Vitro Analysis of E3 Ubiquitin Ligase Function
06:06

In Vitro Analysis of E3 Ubiquitin Ligase Function

Published on: May 14, 2021

6.2K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Cilia and flagella are vital eukaryotic organelles involved in cell motility and environmental sensing.
  • The precise molecular mechanisms governing cilia/flagella assembly, maintenance, disassembly, and signal transduction remain incompletely understood.
  • Post-translational modifications (PTMs), including ubiquitination, are increasingly recognized as critical regulators of these processes.

Purpose of the Study:

  • To review recent advancements in understanding the role of ubiquitination in ciliary and flagellar biology.
  • To summarize the ubiquitin conjugation system components identified in cilia/flagella.
  • To explore the involvement of ciliary/flagellar protein ubiquitination in flagellar disassembly, motility, and signal transduction.

Main Methods:

  • Proteomic analysis to identify ubiquitination system components.
  • Literature review of studies investigating ubiquitination in cilia and flagella.
  • Synthesis of current research findings on the functional roles of ubiquitination.

Main Results:

  • Identification of specific ubiquitin conjugation system elements within cilia/flagella.
  • Demonstration of ubiquitination's involvement in regulating flagellar disassembly.
  • Evidence linking ubiquitination to modulation of ciliary/flagellar motility and signal transduction pathways.

Conclusions:

  • Ubiquitination is a significant PTM impacting multiple facets of ciliary and flagellar function.
  • Further investigation into the specific E3 ligases and deubiquitinating enzymes acting on ciliary/flagellar substrates is warranted.
  • Understanding ciliary/flagellar protein ubiquitination offers potential insights into human diseases associated with ciliopathies.