Multiplex biomarker analysis biosensor for detection of hepatitis B virus

Area of Science:

• Biomedical Engineering
• Immunotechnology
• Viral Diagnostics

Background:

• Hepatitis B virus (HBV) infection is a significant global health concern.
• Current diagnostic methods for HBV serological markers are often time-consuming and detect markers individually.

Purpose of the Study:

• To develop and optimize a protein microarray-based biosensor for simultaneous detection of multiple HBV serological markers.
• To evaluate the performance of the biosensor in terms of sensitivity, specificity, and speed compared to existing methods.

Main Methods:

• Development of a surface plasmon resonance (SPR) based protein microarray.
• Optimization of printing buffer, protein immobilization time, and capture protein concentration.
• Simultaneous detection of five HBV markers in human serum samples.

Main Results:

• The optimized biosensor detected five HBV markers in 20 μL of human serum within 30 minutes.
• Achieved a sensitivity of 0.1 ng/mL for Hepatitis B surface antigen (HBsAg).
• Demonstrated 85% agreement with commercial ELISA kits in analyzing 35 patient samples, showing high specificity, reproducibility, and stability.

Conclusions:

• The integrated protein microarray and SPR technique provides a rapid, sensitive, and specific platform for HBV diagnosis.
• This high-throughput method offers a significant advancement over conventional ELISA and PCR techniques for HBV marker detection.