Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Molecular Hybrids of Serum Albumin and Cobalt Phthalocyanine for Asymmetric Oxidation of C=C and C-H Bonds.

ACS applied materials & interfaces·2026
Same author

Platelet-Prostate cancer crosstalk driving disparities and therapeutic vulnerabilities.

Discover oncology·2026
Same author

ECL-CRISPR array for multiplexed detection of miRNAs.

Biosensors & bioelectronics·2025
Same author

Multiplexed CRISPR Assay for Amplification-Free Detection of miRNAs.

Biosensors·2025
Same author

Investigation of Genomic and Transcriptomic Risk Factors of Clopidogrel Response in African Americans.

Clinical pharmacology and therapeutics·2025
Same author

Transcriptomic and functional characterization of megakaryocytic-derived platelet-like particles: impaired aggregation and prominent anti-tumor effects.

Platelets·2025

Related Experiment Video

Updated: Apr 3, 2026

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
08:22

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

12.7K

3D-printed supercapacitor-powered electrochemiluminescent protein immunoarray.

Karteek Kadimisetty1, Islam M Mosa2, Spundana Malla1

  • 1Department of Chemistry, University of Connecticut, Storrs, CT 06269, USA.

Biosensors & Bioelectronics
|September 26, 2015
PubMed
Summary
This summary is machine-generated.

A 3D-printed, supercapacitor-powered electrochemiluminescent (ECL) protein immunoarray offers a low-cost, sensitive method for detecting prostate cancer biomarkers in serum within 35 minutes. This innovative immunosensor shows promise for rapid, onsite diagnostics, even in resource-limited settings.

Keywords:
3D-printingBiomarker proteinsECL immunoarrayMicrofluidicsProstate cancerSupercapacitor

More Related Videos

Bridging the Bio-Electronic Interface with Biofabrication
16:38

Bridging the Bio-Electronic Interface with Biofabrication

Published on: June 6, 2012

17.4K
Covalent Binding of Antibodies to Cellulose Paper Discs and Their Applications in Naked-eye Colorimetric Immunoassays
09:04

Covalent Binding of Antibodies to Cellulose Paper Discs and Their Applications in Naked-eye Colorimetric Immunoassays

Published on: October 21, 2016

15.4K

Related Experiment Videos

Last Updated: Apr 3, 2026

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
08:22

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

12.7K
Bridging the Bio-Electronic Interface with Biofabrication
16:38

Bridging the Bio-Electronic Interface with Biofabrication

Published on: June 6, 2012

17.4K
Covalent Binding of Antibodies to Cellulose Paper Discs and Their Applications in Naked-eye Colorimetric Immunoassays
09:04

Covalent Binding of Antibodies to Cellulose Paper Discs and Their Applications in Naked-eye Colorimetric Immunoassays

Published on: October 21, 2016

15.4K

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Oncology Diagnostics

Background:

  • Early cancer biomarker detection is crucial for effective treatment.
  • Existing diagnostic methods can be costly, time-consuming, and require specialized facilities.
  • There is a need for rapid, sensitive, and affordable diagnostic tools, particularly for resource-limited settings.

Purpose of the Study:

  • To develop a low-cost, sensitive electrochemiluminescent (ECL) protein immunoarray for cancer biomarker detection.
  • To fabricate the immunosensor using an inexpensive 3D printer and supercapacitor power.
  • To evaluate the device's performance in detecting prostate cancer biomarkers in serum rapidly.

Main Methods:

  • Fabrication of a 3D-printed immunoarray with screen-printed carbon sensors and gravity flow system.
  • Utilizing a sandwich immunoassay with antibody-coated sensors and Ru(bpy)3(2+)-doped silica nanoparticles.
  • Employing supercapacitor power for ECL generation and a CCD camera for detection, with solar cell recharging.

Main Results:

  • The immunosensor detected three prostate cancer biomarkers (PSA, PSMA, PF-4) in serum within 35 minutes.
  • Achieved detection limits of 300-500 fg/mL for the biomarkers in undiluted calf serum.
  • Demonstrated good correlation with conventional ELISAs using patient serum samples.

Conclusions:

  • The 3D-printed ECL immunoarray is a cost-effective and sensitive platform for rapid cancer biomarker detection.
  • The technology is suitable for onsite diagnostics in resource-limited environments with minimal training.
  • This approach offers a promising alternative for early cancer screening and diagnosis.