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Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
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Comparison of Whole-Genome Sequencing and Molecular-Epidemiological Techniques for Clostridium difficile Strain
Samuel R Dominguez1, Lydia J Anderson2, Cassandra V Kotter3
1Department of Pediatric Infectious Diseases, University of Colorado School of Medicine, Aurora Department of Epidemiology, Children's Hospital Colorado, Aurora.
Abstract:
We analyzed in parallel 27 pediatric Clostridium difficile isolates by repetitive sequence-based polymerase chain reaction (RepPCR), pulsed-field gel electrophoresis (PFGE), and whole-genome next-generation sequencing. Next-generation sequencing distinguished 3 groups of isolates that were indistinguishable by RepPCR and 1 isolate that clustered in the same PFGE group as other isolates.
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