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Morphometric Protocol for the Objective Assessment of Blastocyst Behavior During Vitrification and Warming Steps
Published on: February 28, 2019
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Morphokinetics analysis of embryos derived from vitrified/warmed oocytes
D Montjean1, C Geoffroy-Siraudin2, M Gervoise-Boyer2
1Service de Médecine et Biologie de la Reproduction, Hôpital Saint-Joseph, 26 boulevard de Louvain, 13008, Marseille, France. debbie_montjean@hotmail.com.
Journal of Assisted Reproduction and Genetics
|September 28, 2015
Summary
Oocyte vitrification does not significantly impact embryo development. This study found no major differences in cleavage rates, blastomere size, or blastocyst formation between embryos from vitrified/warmed oocytes and fresh sibling oocytes.
Area of Science:
- Reproductive biology
- Embryology
- Assisted reproductive technology
Background:
- Oocyte vitrification is a widely adopted fertility preservation technique.
- While generally preserving oocyte integrity, recent concerns suggest potential impacts on early embryo development.
Purpose of the Study:
- To compare embryo development from vitrified/warmed oocyte-derived embryos (VODE) versus fresh oocyte-derived embryos (FODE).
- To evaluate key developmental parameters including cleavage rate, blastomere size, fragmentation, and blastocyst formation.
Main Methods:
- Included 90 infertility cases with ample mature oocytes.
- Oocytes were divided into fresh and vitrified groups for ICSI.
- Embryo development from zygote to blastocyst was prospectively monitored up to day 5.
Main Results:
- No significant differences were observed between VODE and FODE in cleavage rate, blastomere count, fragmentation, or blastomere size.
- Oocyte vitrification did not affect the percentage of morulae at day 4 or blastocysts at day 5.
Conclusions:
- Oocyte vitrification does not appear to alter early embryo development.
- Long-term offspring follow-up is recommended to confirm the absence of adverse developmental effects.
