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Related Experiment Video

Updated: Apr 3, 2026

Monitoring Stub1-Mediated Pexophagy

Monitoring Stub1-Mediated Pexophagy

Published on: May 12, 2023

Pexophagy in yeasts.

Masahide Oku¹, Yasuyoshi Sakai²

¹Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Japan.

Biochimica Et Biophysica Acta

|September 28, 2015

Summary

Pexophagy, the selective removal of peroxisomes, is crucial for cell health. Research in yeast has uncovered key molecular players, especially adaptor proteins, that drive this essential autophagic process.

Keywords:

Autophagy Peroxisomes Vacuole Yeast

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A Fluorescence Microscopy Assay for Monitoring Mitophagy in the Yeast Saccharomyces cerevisiae

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Published on: July 18, 2011

Area of Science:

Cell Biology
Molecular Biology
Autophagy Research

Background:

Pexophagy is the primary mechanism for reducing peroxisome numbers in cells.
Studies in yeast, including Saccharomyces cerevisiae, Pichia pastoris, and Hansenula polymorpha, have been instrumental in understanding pexophagy.
The molecular machinery governing pexophagy is complex and involves specific protein interactions.

Purpose of the Study:

To elucidate the molecular machinery of pexophagy in yeast.
To detail the roles of adaptor proteins in guiding autophagic membrane formation during pexophagy.
To discuss the functional relevance of pexophagy and mitophagy, and the physiological importance of pexophagy in yeast.

Main Methods:

Comparative analysis of pexophagy mechanisms across different yeast species.
Investigation of protein interactions and modifications involved in pexophagy.
Review and synthesis of recent findings on yeast autophagy.

Main Results:

The molecular machineries of pexophagy have been significantly elucidated in yeast.
Adaptor proteins play a critical role in directing autophagic membrane biogenesis to peroxisomes.
Interactions and modifications of these adaptor proteins are key to pexophagy regulation.

Conclusions:

Recent yeast studies have revealed the intricate molecular details of pexophagy.
Understanding pexophagy provides insights into organelle homeostasis and cellular quality control.
Pexophagy is physiologically important in yeast, alongside other autophagic pathways like mitophagy.