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Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
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Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
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The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System
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Rat full term amniotic fluid harbors highly potent stem cells.

Hoo Mun-Fun1, Nurfarhana Ferdaos2, Siti Nurusaadah Hamzah1

  • 1Genetics and Regenerative Medicine Research Centre, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor Darul Ehsan, Malaysia; Department of Obstetrics & Gynecology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor Darul Ehsan, Malaysia.

Research in Veterinary Science
|September 29, 2015
PubMed
Summary

Full-term amniotic fluid contains potent stem cells. These amniotic fluid stem cells (AFSCs) show pluripotency and neurogenic properties, offering a promising source for regenerative medicine.

Keywords:
Amniotic fluid stem cells (AFSCs)Broad multipotent stem cellsEmbryoid bodiesFull term amniotic fluidNeurogenic potentialPluripotency testPotentially pluripotent

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Area of Science:

  • Stem cell biology
  • Developmental biology
  • Regenerative medicine

Background:

  • Amniotic fluid stem cells (AFSCs) are typically obtained via invasive amniocentesis.
  • The presence and characteristics of AFSCs in full-term amniotic fluid remain largely unexplored.

Purpose of the Study:

  • To isolate and characterize stem cells from the amniotic fluid of full-term pregnant rats.
  • To evaluate the stemness and differentiation potential of these cells for regenerative applications.

Main Methods:

  • Immuno-selection using the c-kit marker to isolate stem cell lines from full-term rat amniotic fluid.
  • Assessment of pluripotency markers, embryoid body formation, and spontaneous/directed differentiation.
  • Long-term culture to evaluate cell line stability.

Main Results:

  • Three stem cell lines were successfully established.
  • Two lines maintained pluripotency markers past passage 90.
  • Cells formed high-quality embryoid bodies and differentiated into neural lineages, demonstrating multi-lineage potential.

Conclusions:

  • Full-term amniotic fluid harbors highly potent stem cells.
  • These stem cells possess significant stemness and neurogenic capabilities.
  • AFSCs from full-term pregnancies represent a viable, non-invasive source for regenerative medicine.