Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Ion-Exchange Chromatography

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Synergistic effect of plunger insertion and siliconization methods on particle formation of an antibody formulation.

Journal of pharmaceutical sciences·2025

Same author

A doctor of pharmacy curriculum revision process focused on curricular overload.

American journal of pharmaceutical education·2024

Same author

VDX-111 targets proliferative pathways in canine cancer cell lines.

PloS one·2024

Same author

VDX-111, a novel small molecule, induces necroptosis to inhibit ovarian cancer progression.

Molecular carcinogenesis·2024

Same author

Comparative Thermodynamics of the Reversible Self-Association of Therapeutic mAbs Reveal Opposing Roles for Linked Proton- and Ion-Binding Events.

Pharmaceutical research·2023

Same author

Expression Differences in BCL2 Family Members between Uveal and Cutaneous Melanomas Account for Varying Sensitivity to BH3 Mimetics.

The Journal of investigative dermatology·2021

Search research articles

Related Experiment Video

Updated: Apr 2, 2026

Biochemical Reconstitution of Steroid Receptor•Hsp90 Protein Complexes and Reactivation of Ligand Binding

Biochemical Reconstitution of Steroid Receptor•Hsp90 Protein Complexes and Reactivation of Ligand Binding

Published on: September 21, 2011

Dissecting Steroid Receptor Function by Analytical Ultracentrifugation.

David L Bain¹, Rolando W De Angelis¹, Keith D Connaghan¹

¹Department of Pharmaceutical Sciences, University of Colorado Anschutz Medical Campus, Aurora, Colorado, USA.

Methods in Enzymology

|September 29, 2015

Summary

Steroid receptors, including androgen receptor (AR) and estrogen receptor (ER), control gene expression. This study quantifies their interaction energetics with DNA, revealing mechanisms for specific gene regulation.

Keywords:

Androgen receptor Estrogen receptor Glucocorticoid receptor Nuclear hormone receptors Protein–DNA interactions Thermodynamics Transcriptional regulation

More Related Videos

Purification and Reconstitution of TRPV1 for Spectroscopic Analysis

Purification and Reconstitution of TRPV1 for Spectroscopic Analysis

Published on: July 3, 2018

Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain

Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain

Published on: December 12, 2017

Related Experiment Videos

Last Updated: Apr 2, 2026

Biochemical Reconstitution of Steroid Receptor•Hsp90 Protein Complexes and Reactivation of Ligand Binding

Biochemical Reconstitution of Steroid Receptor•Hsp90 Protein Complexes and Reactivation of Ligand Binding

Published on: September 21, 2011

Purification and Reconstitution of TRPV1 for Spectroscopic Analysis

Purification and Reconstitution of TRPV1 for Spectroscopic Analysis

Published on: July 3, 2018

Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain

Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain

Published on: December 12, 2017

Area of Science:

Molecular biology
Biochemistry
Genetics

Background:

Steroid receptors are ligand-activated transcription factors regulating key biological processes.
While qualitative understanding of gene regulation is established, quantitative mechanisms remain unclear.
Receptors include androgen receptor (AR), estrogen receptor (ER), glucocorticoid receptor (GR), mineralocorticoid receptor (MR), and progesterone receptor (PR).

Purpose of the Study:

To delineate the physical mechanisms of steroid receptor-mediated gene activation.
To systematically dissect receptor interaction energetics with multisite regulatory elements.
To link in vitro findings to in vivo cellular function.

Main Methods:

Analytical ultracentrifugation (AUC) was employed for quantitative analysis.
Sedimentation velocity and sedimentation equilibrium were used to determine receptor assembly states.
Comparative analysis across the steroid receptor family was performed.

Main Results:

AUC revealed the energetics of receptor self-association.
Thermodynamic coupling between receptor self-association and DNA binding was elucidated.
Receptor assembly at multisite regulatory elements was analyzed.

Conclusions:

Findings provide quantitative insight into steroid receptor-mediated gene control.
Receptor assembly dynamics are crucial for specific gene regulation.
The study bridges in vitro biophysical measurements with in vivo cellular receptor function.