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Advancing understanding of microbial bioenergy conversion processes by activity-based protein profiling.

Yun Liu1, James K Fredrickson2, Natalie C Sadler2

  • 1Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, 100029 Beijing, China.

Biotechnology for Biofuels
|September 29, 2015
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Summary

Activity-based protein profiling (ABPP) uses chemical probes to measure enzyme function, accelerating the discovery of microbial biocatalysts for advanced renewable biofuels. This technology is key to improving biofuel production platforms.

Keywords:
Activity-based protein profiling (ABPP)BiodieselCellulosic bioethanolProtein redoxProteomics

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Area of Science:

  • Microbial Systems Biology
  • Biocatalysis
  • Renewable Energy

Background:

  • Global priority on renewable biofuels necessitates advanced microbial understanding.
  • Activity-based protein profiling (ABPP) is a powerful tool for functional enzyme characterization.
  • ABPP enables direct measurement of enzyme activity in vitro and in vivo.

Purpose of the Study:

  • To review the design strategies and recent advances in ABPP for biofuels.
  • To highlight the pivotal role of ABPP in cellulosic bioethanol, biodiesel, and hydrocarbon production.
  • To examine challenges and opportunities for ABPP in renewable biofuels research.

Main Methods:

  • Review of general ABPP design strategies.
  • Highlighting recent ABPP advances applicable to biofuels.
  • Examining applications in cellulosic bioethanol, biodiesel, and phototrophic hydrocarbon production.

Main Results:

  • ABPP facilitates rapid discovery of novel microbial biocatalysts.
  • ABPP can significantly improve biofuel production platforms.
  • Recent advances show ABPP's potential in diverse biofuel applications.

Conclusions:

  • ABPP is crucial for advancing renewable biofuels through enhanced microbial systems biology.
  • Integrating ABPP with other biological approaches offers new insights into enzyme mechanisms.
  • ABPP is a key technology for optimizing biofuel production processes.