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Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
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Subangstrom single-molecule measurements of motor proteins using a nanopore
Ian M Derrington1, Jonathan M Craig1, Eric Stava2
1Department of Physics, University of Washington, Seattle, Washington, USA.
Nature Biotechnology
|September 29, 2015
Abstract:
Techniques for measuring the motion of single motor proteins, such as FRET and optical tweezers, are limited to a resolution of ∼300 pm. We use ion current modulation through the protein nanopore MspA to observe translocation of helicase Hel308 on DNA with up to ∼40 pm sensitivity. This approach should be applicable to any protein that translocates on DNA or RNA, including helicases, polymerases, recombinases and DNA repair enzymes.
