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ELISA with enzyme amplification for sensitive detection of staphylococcal enterotoxins in food
H Windemann1, J Lüthy, M Maurer
1Institute of Biochemistry, University of Bern, Switzerland.
International Journal of Food Microbiology
|February 1, 1989
Abstract:
A highly sensitive amplified ELISA in microtitre plates for the detection of staphylococcal enterotoxins compared favourably with a commercially available kit. The amplified ELISA demonstrated a reduced effect of the food sample matrix and an ease of handling. Using the amplified ELISA, the influence of different parameters (pH, time, temperature, re-extraction, concentration procedure) on the recovery efficiency and assay reliability was tested on foods implicated in food poisoning outbreaks as well as on spiked foods containing low levels of enterotoxins.