Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.9K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.9K
Spectrophotometry: Introduction01:16

Spectrophotometry: Introduction

11.1K
Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
11.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Combination therapy for exacerbations of pain in osteoarthritis with non-fixed combinations].

Terapevticheskii arkhiv·2025
Same author

A Method for Assessing Working Memory in Rats Using Controlled Virtual Environment.

Sovremennye tekhnologii v meditsine·2024
Same author

[Vitamin B12 deficiency in the elderly and its associations with geriatric syndromes].

Zhurnal nevrologii i psikhiatrii imeni S.S. Korsakova·2024
Same author

Clinical Manifestations and Prognosis of Giant Cell Arteritis: A Retrospective Cohort Study.

Doklady. Biochemistry and biophysics·2024
Same author

[Real-world experience with netakimab in the treatment of spondyloarthritis].

Terapevticheskii arkhiv·2024
Same author

[Personalized patient-oriented approach to the treatment of musculoskeletal pain syndrome using over-the-counter medications. Resolution of the Expert Council (May 28, 2023)].

Zhurnal nevrologii i psikhiatrii imeni S.S. Korsakova·2023

Related Experiment Video

Updated: Apr 2, 2026

Spectral Reflectometric Microscopy on Myelinated Axons In Situ
09:13

Spectral Reflectometric Microscopy on Myelinated Axons In Situ

Published on: July 2, 2018

7.8K

Single-molecule spectromicroscopy: a route towards sub-wavelength refractometry.

T A Anikushina1, M G Gladush, A A Gorshelev

  • 1Molecular Spectroscopy Dept., Institute for Spectroscopy of the Russian Academy of Sciences, Fizicheskaya Str 5, Troitsk, Moscow, 142190 Russia. a_v_naumov@mail.ru.

Faraday Discussions
|September 29, 2015
PubMed
Summary

Single-molecule spectroscopy reveals local refractive index fluctuations in solids. This method uses dye molecule lifetime changes to map these variations, aiding materials science research.

More Related Videos

Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing
08:12

Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing

Published on: March 13, 2013

13.3K
A Multimodal Wide-Field Fourier-Transform Raman Microscope
06:48

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

752

Related Experiment Videos

Last Updated: Apr 2, 2026

Spectral Reflectometric Microscopy on Myelinated Axons In Situ
09:13

Spectral Reflectometric Microscopy on Myelinated Axons In Situ

Published on: July 2, 2018

7.8K
Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing
08:12

Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing

Published on: March 13, 2013

13.3K
A Multimodal Wide-Field Fourier-Transform Raman Microscope
06:48

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

752

Area of Science:

  • Solid-state physics
  • Materials science
  • Spectroscopy

Background:

  • Local fluctuations in refractive index (n) impact material properties.
  • Spatially resolved measurements of local n are challenging.
  • Single-molecule (SM) spectroscopy offers high sensitivity for probing local environments.

Purpose of the Study:

  • To introduce a novel method for spatially resolved probing of local refractive index fluctuations in solids.
  • To demonstrate the utility of single-molecule spectroscopy for this purpose.
  • To investigate the relationship between molecular lifetime and local refractive index.

Main Methods:

  • Utilizing the dependence of effective radiative lifetime (T1) of dye centers on the local refractive index (n).
  • Employing single-molecule spectroscopy to detect zero-phonon lines at low temperatures.
  • Analyzing the natural spectral linewidth of single molecules, which is inversely proportional to T1.

Main Results:

  • Demonstrated the possibility of revealing the distribution of local refractive index values in solids.
  • Applied the method to amorphous polyethylene and polycrystalline naphthalene doped with terrylene.
  • Showed that lifetime-limited spectral linewidth distributions correlate with local refractive index fluctuations.

Conclusions:

  • The developed single-molecule spectroscopy approach enables spatially resolved mapping of local refractive index variations in solids.
  • This technique provides insights into the heterogeneity of solid materials at the molecular level.
  • The findings are relevant for understanding and manipulating optical properties of materials.