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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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A Microflow Cytometer Based on a Disposable Microfluidic Chip With Side Scatter and Fluorescence Detection

Wenpeng Xun, Jianguo Feng, Honglong Chang

    IEEE Transactions on Nanobioscience
    |September 29, 2015
    PubMed
    Summary

    This study introduces a novel microflow cytometer using a polydimethylsiloxane (PDMS) microfluidic chip. The device demonstrates high precision and successful cell gating, offering a promising solution for point-of-care diagnostics.

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    Area of Science:

    • Biomedical Engineering
    • Microfluidics
    • Flow Cytometry

    Background:

    • Traditional flow cytometers are often large, expensive, and complex.
    • There is a growing need for portable and cost-effective diagnostic tools for point-of-care applications.

    Purpose of the Study:

    • To develop and validate a microflow cytometer based on a polydimethylsiloxane (PDMS) microfluidic chip.
    • To assess the performance of the microflow cytometer for cell counting and analysis.

    Main Methods:

    • Fabrication of a PDMS microfluidic chip using a double molding process for smooth sidewalls.
    • Implementation of a sheathless focusing mechanism to minimize coincidence error.
    • Utilizing side scatter (SSC) and fluorescein isothiocyanate (FITC) channels for detection.
    • Employing Flow-Check alignment beads for calibration and validation.

    Main Results:

    • Achieved a coincidence error of less than 0.069% with the sheathless focus mechanism.
    • Demonstrated coefficients of variation (CV) of 8.37% for SSC and 2.46% for FITC.
    • Successfully performed lymphocyte gating based on CD45/SSC markers.

    Conclusions:

    • The developed PDMS microfluidic chip enables effective side scatter detection, crucial for cytometer functionality.
    • The microflow cytometer exhibits high precision and accuracy, suitable for cell analysis.
    • This technology presents a viable and promising solution for developing point-of-care microflow cytometers.