[Determination of Dencichine and Its Isomer in Panax notoginseng by Pre-column Derivatization HPLC]

To establish a method to determine dencichine (β-ODAP) and its isomer (α-ODAP) in Panax notoginseng by pre-column derivatization HPLC with 1-fluoro-2,4-dinitrobenzene (FDNB).

A Luna-C18 column (250 mm x 4.6 mm, 5 µm) was used, acetonitrile- (HAc-NaAc) as the mobile phase with the ratio of 17:83, the detection wavelength was 360 nm, the column temperature was 40 °C, and 20 µL of sample was injected for HPLC analysis.

In 0.30-50.50 µg/mL,β-ODAP had a good linear relationship with peak area, A2 = 140.50C1 + 72.30, r1 = 0.9995; In 0.10-16.15 µg/mL, α-ODAP has a good linear relationship with peak area, A2 = 106.60C2 + 56.00, r2 = 0.9992.

There are appreciable β-ODAP and α-ODAP in different samples of Panax notoginseng. The method is simple, rapid, accurate and can be used for the detection of dencichine and its isomer in other samples.