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Related Concept Videos

Spermatogenesis01:41

Spermatogenesis

124.7K
Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
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Spermatogenesis01:22

Spermatogenesis

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Spermatogenesis is a complex process that involves the development of sperm cells from undifferentiated stem cells in the seminiferous tubules of the testes. The process is essential for the production of mature and functional sperm cells that are capable of fertilizing an egg.
The process of spermatogenesis can be divided into mitosis, meiosis, and spermiogenesis. During mitosis, the spermatogonia or stem cells divide to produce two identical daughter cells, type A and B spermatogonia. Type-A...
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Proteomics01:33

Proteomics

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A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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Related Experiment Video

Updated: Apr 2, 2026

Isolation of Murine Spermatogenic Cells using a Violet-Excited Cell-Permeable DNA Binding Dye
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Linking transcriptomics and proteomics in spermatogenesis.

Frédéric Chalmel1, Antoine D Rolland1

  • 1Inserm U1085-IrsetUniversité de Rennes 1, F-35042 Rennes, France frederic.chalmel@inserm.fr antoine.rolland@univ-rennes1.fr.

Reproduction (Cambridge, England)
|September 30, 2015
PubMed
Summary

This review explores the link between gene expression (transcription) and protein production (translation) during sperm development. It highlights new methods for integrating transcriptomic and proteomic data to understand male fertility.

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Area of Science:

  • Reproductive Biology
  • Molecular Biology
  • Genomics

Background:

  • Spermatogenesis, the production of male gametes, is a complex process regulated by gene expression.
  • Understanding the molecular basis of spermatogenesis is crucial for addressing male infertility.
  • High-throughput studies have investigated genes and proteins involved in male fertility.

Purpose of the Study:

  • To review studies correlating transcription and translation during spermatogenesis.
  • To discuss advanced transcriptomic methods as proxies for the proteome.
  • To illustrate data integration for novel discoveries in male gamete development.

Main Methods:

  • Comparative analysis of testicular transcriptome and proteome.
  • Evaluation of new transcriptomic approaches (e.g., RNA-seq).
  • Integration of transcriptomic and MS-based proteomics data.

Main Results:

  • Identified challenges and advancements in correlating gene and protein expression in spermatogenesis.
  • Highlighted the utility of transcriptomic data to inform proteomic studies.
  • Demonstrated the potential for discovering new genes and protein isoforms.

Conclusions:

  • Integrating transcriptomic and proteomic data offers powerful insights into spermatogenesis.
  • Advanced transcriptomic techniques improve the understanding of the proteome.
  • 'Proteomics informed by transcriptomics' aids in discovering novel molecular players in male fertility.