Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Mutagenicity and Carcinogenicity

Mutagenicity and Carcinogenicity

Mutagenicity and carcinogenicity refer to the ability of drugs to cause genetic defects and induce cancer, respectively. The International Agency for Research on Cancer (IARC) classifies agents into four groups based on their carcinogenic potential. Group 1 agents are known human carcinogens; group 2A agents are probably carcinogenic to humans; group 3 agents lack data to support their role in carcinogenesis; and group 4 includes agents for which data support that they are not likely to be...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Lentiviral-mediated expression of cytochrome P450 2D6 in HepaRG cells: new means for in vitro xenobiotic biotransformation and cellular response to chimeric viral mRNA.

Archives of toxicology·2026

Same author

Iron deficiency induces maturation-dependent loss of pancreatic β-cells.

Nature communications·2026

Same author

The clinically available supplement pyruvate enhances the therapeutic effect of bortezomib in Multiple Myeloma by modulating mitochondrial metabolism.

Cancer letters·2026

Same author

Effects of physiological oxygen tension on human cumulus-oocyte-complex metabolism during in vitro maturation: an exploratory study.

Journal of ovarian research·2025

Same author

Intact corticostriatal function in aged system x<sub>c</sub><sup>-</sup> - deficient mice.

Translational psychiatry·2025

Same author

An islet-resident macrophage antioxidant program preserves β cell physiology.

Science immunology·2025

Search research articles

Related Experiment Video

Updated: Apr 1, 2026

High Content Screening Analysis to Evaluate the Toxicological Effects of Harmful and Potentially Harmful Constituents HPHC

High Content Screening Analysis to Evaluate the Toxicological Effects of Harmful and Potentially Harmful Constituents HPHC

Published on: May 10, 2016

Testing chemical carcinogenicity by using a transcriptomics HepaRG-based model?

T Y Doktorova¹, Reha Yildirimman², Liesbeth Ceelen³

¹Vrije Universiteit Brussel, Department of Toxicology, Center for Pharmaceutical Research, Brussels, Belgium.

|September 30, 2015

Summary

This summary is machine-generated.

The carcinoGENOMICS project successfully combined toxicogenomics with HepaRG cells to distinguish genotoxic from non-genotoxic carcinogens. This transcriptomics approach shows promise for evaluating chemical genotoxicity.

Keywords:

HepaRG cell line gene expression profiling genotoxic carcinogens liver-based in vitro models non-genotoxic carcinogens pathways-based analysis

More Related Videos

Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure

Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure

Published on: June 5, 2020

An Oncogenic Hepatocyte-Induced Orthotopic Mouse Model of Hepatocellular Cancer Arising in the Setting of Hepatic Inflammation and Fibrosis

An Oncogenic Hepatocyte-Induced Orthotopic Mouse Model of Hepatocellular Cancer Arising in the Setting of Hepatic Inflammation and Fibrosis

Published on: September 12, 2019

Related Experiment Videos

Last Updated: Apr 1, 2026

High Content Screening Analysis to Evaluate the Toxicological Effects of Harmful and Potentially Harmful Constituents HPHC

High Content Screening Analysis to Evaluate the Toxicological Effects of Harmful and Potentially Harmful Constituents HPHC

Published on: May 10, 2016

Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure

Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure

Published on: June 5, 2020

An Oncogenic Hepatocyte-Induced Orthotopic Mouse Model of Hepatocellular Cancer Arising in the Setting of Hepatic Inflammation and Fibrosis

An Oncogenic Hepatocyte-Induced Orthotopic Mouse Model of Hepatocellular Cancer Arising in the Setting of Hepatic Inflammation and Fibrosis

Published on: September 12, 2019

Area of Science:

Toxicogenomics
In vitro toxicology
Carcinogenesis research

Background:

The EU FP6 project carcinoGENOMICS aimed to integrate toxicogenomics with in vitro cell models.
Identifying organ-specific gene signatures for genotoxic and non-genotoxic carcinogens was a key objective.

Purpose of the Study:

To report the performance of a gene classifier developed using HepaRG cells.
To evaluate the classifier's ability to distinguish between genotoxic and non-genotoxic compounds.

Main Methods:

HepaRG cells were exposed to 30 prototypical compounds (10 non-carcinogens, 20 hepatocarcinogens).
Gene expression data were analyzed using biostatistical approaches at gene and pathway levels.
Interlaboratory reproducibility was assessed through blind testing of three compounds by three independent labs.

Main Results:

The gene classifier achieved up to 88% correct prediction, distinctly separating genotoxic from non-genotoxic hepatocarcinogens and non-carcinogens.
The DNA damage pathway was the most characteristic response to genotoxic exposure.
Interlaboratory testing confirmed correct prediction of genotoxicants, though predictability was lower for non-genotoxic compounds.

Conclusions:

The integration of transcriptomics and the HepaRG in vitro model offers a potential weight-of-evidence approach.
This method can aid in evaluating the genotoxic potential of chemical substances.
The study highlights the utility of toxicogenomics in carcinogenicity assessment.