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Updated: Apr 1, 2026

Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling
Published on: February 5, 2018
Multivalent presentation of carbohydrates by 3(14)-helical peptide templates: synthesis, conformational analysis
Nitin J Pawar1, Ulf Diederichsen, Dilip D Dhavale
1Department of Chemistry, Garware Research Centre, Savitribai Phule Pune University (Formerly University of Pune), Pune - 411 007, India. ddd@chem.unipune.ac.in.
Abstract:
A well defined 314-helical tetravalent β-galactopeptide site-specific functionalised template (SSFT) 1 was prepared containing d-galactose units, with free anomeric carbons as the aldehyde tags, and was explored via ligation with different aminoxy sugars (α-/β-d-glucose, α/β-d-galactose, α-d-mannose and β-d-lactose) to get 314-helical carbohydrate-functionalised multivalent glycoconjugates 2-7. Preliminary recognition studies of tetramannosyl glycoconjugate 4 with a specific lectin (concanavalin A) using fluorescence anisotropy showed an increase in binding affinity and the multivalency effect was found to be increased by 6.5 times per glycan.
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