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Updated: Apr 1, 2026
Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies
Published on: May 6, 2022
DNA 5-hydroxymethylation in human adipose tissue differs between subcutaneous and visceral adipose tissue depots
Kerstin Rohde1, Maria Keller1, Michael Stumvoll1,2
1IFB Adiposity Diseases, University of Leipzig, Leipzig, Germany.
Background:
A stable intermediate during DNA demethylation, 5-hydroxymethylcytosine (5-hmC), raises questions about its function and distribution. Therefore, we tested whether 5-hmC exists in human adipose tissue depots and correlates with clinical variables.
Materials & Methods:
We measured the % 5-hmC content in both subcutaneous adipose tissue and visceral adipose tissue (VAT) from 81 individuals by using ELISA technology. To test for associations with several clinical variables we used paired students t-tests and linear regression analyses.
Results:
We observed an average % 5-hmC content of 0.47% ± 0.093 in subcutaneous adipose tissue, while VAT (0.51% ± 0.122) is higher hydroxymethylated (p = 0.005). In the total cohort we observed a positive association of % 5-hmC in VAT with age (p = 0.034) and a negative relationship with low density lipoprotein-cholesterol (p = 0.008).
Conclusion:
Our data suggest adipose tissue depot specific 5-hmC levels with higher levels in VAT.
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