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A Detailed Protocol for Perspiration Monitoring Using a Novel, Small, Wireless Device

A Detailed Protocol for Perspiration Monitoring Using a Novel, Small, Wireless Device

Published on: November 24, 2016

Flexible nanoporous tunable electrical double layer biosensors for sweat diagnostics.

Rujuta D Munje¹, Sriram Muthukumar², Anjan Panneer Selvam¹

¹Department of Bioengineering, University of Texas at Dallas, 800 W. Campbell Road, EC 39, Richardson, TX 75080.

Scientific Reports

|October 1, 2015

Summary

This study presents a novel wearable biosensor for detecting the stress biomarker cortisol in sweat. The ultra-sensitive device utilizes an electrical double layer effect for highly specific and accurate stress monitoring.

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Area of Science:

Biomedical Engineering
Nanotechnology
Analytical Chemistry

Background:

Wearable diagnostics require sensitive and specific detection of biomarkers.
Cortisol is a key stress biomarker, and its non-invasive monitoring is highly desirable.
Existing methods for cortisol detection often lack the sensitivity or portability for continuous monitoring.

Purpose of the Study:

To develop an ultra-sensitive and highly specific electrical double layer (EDL) modulated biosensor for wearable diagnostics.
To detect the stress biomarker cortisol in synthetic and human sweat.
To utilize nanoporous flexible substrates for enhanced biosensor performance.

Main Methods:

Fabrication of a biosensor using a zinc oxide thin film on a nanoporous flexible substrate.
Detection of cortisol by measuring impedance changes modulated by the electrical double layer (EDL).
Application of a low orthogonally directed alternating current (AC) electric field to amplify EDL capacitance changes.

Main Results:

Demonstrated high sensitivity for cortisol detection: 1 pg/mL in synthetic sweat and 1 ng/mL in human sweat.
Achieved high specificity, with IL-1β used as a control in synthetic sweat.
Successfully detected cortisol in human sweat over a concentration range of 10-200 ng/mL.

Conclusions:

The developed EDL-modulated biosensor offers a promising platform for sensitive and specific wearable cortisol monitoring.
Nanoporous flexible substrates significantly enhance EDL formation, improving biosensor performance.
This technology enables non-invasive, real-time stress level assessment through sweat analysis.