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Related Experiment Video

Updated: Apr 1, 2026

Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain

Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain

Published on: July 12, 2012

Analysis of DNA Methylation by Pyrosequencing.

Colin Delaney¹, Sanjay K Garg¹, Raymond Yung²

¹Department of Internal Medicine, University of Michigan Medical School, 109 Zina Pitcher Place, Ann Arbor, MI, 48109, USA.

Methods in Molecular Biology (Clifton, N.J.)

|October 1, 2015

Summary

This summary is machine-generated.

Pyrosequencing enables real-time DNA methylation analysis, crucial for understanding aging and diseases like cancer. This technique quantifies DNA methylation patterns, aiding in early disease detection and identifying potential drug targets.

Keywords:

Assay design Biomarker detection Bisulfide conversation DNA CpG methylation Global methylation Pyrosequencing technique SNPs

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Last Updated: Apr 1, 2026

Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain

Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain

Published on: July 12, 2012

Targeted DNA Methylation Analysis by Next-generation Sequencing

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

DNA Methylation: Bisulphite Modification and Analysis

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

Area of Science:

Molecular Biology
Epigenetics
Genomics

Background:

DNA methylation alterations are linked to aging and various diseases, including cancer, cardiovascular, neurodegenerative, and autoimmune conditions.
Quantitative analysis of DNA CpG methylation is essential for understanding gene regulation in pathophysiological states.

Purpose of the Study:

To provide a detailed technical protocol for pyrosequencing.
To offer critical information on assay design and system nuances for beginners in DNA methylation analysis.

Main Methods:

Utilizing pyrosequencing, a sequencing-by-synthesis method, to quantify single-nucleotide polymorphisms (SNPs).
Employing artificial C/T SNP creation via bisulfite modification for real-time local and global DNA methylation measurement.

Main Results:

The study details a robust protocol for pyrosequencing-based DNA methylation analysis.
The protocol facilitates accurate quantification of DNA methylation patterns at both global and gene-specific levels.

Conclusions:

Pyrosequencing offers a powerful tool for detecting and quantifying DNA methylation patterns.
This technique has the potential to serve as an early diagnostic marker and a target for therapeutic intervention in various diseases.