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Related Experiment Video

Updated: Apr 1, 2026

Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination
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Histone modification and chromatin remodeling during NER.

Raymond Waters1, Patrick van Eijk2, Simon Reed2

  • 1Institute of Cancer and Genetics, School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, UK; College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP, UK.

DNA Repair
|October 1, 2015
PubMed
Summary
This summary is machine-generated.

Histone acetylation, particularly by Gcn5, is crucial for efficient global genome nucleotide excision repair (GG-NER) in yeast. This process involves factors like Rad16 and histone variants, impacting DNA repair accuracy.

Keywords:
ChromatinGlobal genome nucleotide excision repairHistone acetylationHistone variant H2AXRad16Saccharomyces cerevisiaeUV-induced cyclobutane pyrimidine dimers

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Area of Science:

  • Molecular Biology
  • Genetics
  • Epigenetics

Background:

  • DNA damage repair mechanisms are essential for maintaining genomic stability.
  • Nucleotide Excision Repair (NER) is a major pathway for removing bulky DNA lesions.
  • Chromatin structure significantly influences DNA repair accessibility and efficiency.

Purpose of the Study:

  • To review developments in high-resolution studies of global genome nucleotide excision repair (GG-NER) in Saccharomyces cerevisiae.
  • To investigate the relationship between GG-NER and histone modifications, specifically acetylation.
  • To develop and apply high-resolution, genome-wide approaches for studying NER and chromatin interactions.

Main Methods:

  • Development of technologies for nucleotide-resolution examination of NER.
  • Analysis of NER in relation to local chromatin changes and histone acetylation.
  • Utilizing model genes (MFA2, URA3) and genome-wide approaches to study DNA repair.

Main Results:

  • Identified histone acetyltransferase Gcn5 and histone H3 acetylation (lysines 9/14) as key factors for efficient GG-NER.
  • Demonstrated the influence of factors such as Rad16 and Htz1 on Gcn5-mediated repair.
  • Observed a role for histone H4 acetylation in subtelomeric regions (URA3).

Conclusions:

  • Histone acetylation is a critical regulator of GG-NER efficiency in yeast.
  • Specific histone modifications and chromatin remodelers facilitate DNA damage repair.
  • High-resolution, genome-wide approaches are advancing the understanding of chromatin dynamics in DNA repair.