Related Experiment Video
Updated: Apr 1, 2026
08:40
Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging
Published on: April 8, 2016
13.5K
AMBRA1 and SQSTM1 expression pattern in prostate cancer
Laura Falasca1, Francesco Torino2, Matteo Marconi3
1Laboratory of Cell Biology and Electron Microscopy, National Institute for Infectious Diseases I.R.C.C.S. 'L. Spallanzani', Rome, Italy.
Apoptosis : an International Journal on Programmed Cell Death
|October 2, 2015
Summary
Prostate cancer cells show defective autophagy, accumulating proteins AMBRA1 and SQSTM1. Increased AMBRA1 correlates with higher Gleason scores, suggesting its role in cancer progression.
Area of Science:
- Oncology
- Cell Biology
- Molecular Pathology
Background:
- Prostate cancer is a leading cause of male cancer mortality.
- Autophagy is implicated in malignant cell survival and drug resistance in prostate cancer.
Purpose of the Study:
- To investigate the expression of AMBRA1 and sequestosome-1 (SQSTM1) in prostate cancer.
- To evaluate the association between these proteins and the autophagic process in prostate cancer.
- To explore AMBRA1 as a potential biomarker for prostate cancer progression.
Main Methods:
- Immunohistochemistry and western blot analysis of benign and malignant prostatic tissue samples.
- Evaluation of AMBRA1, SQSTM1, and LC3II expression.
- Correlation analysis with Gleason score.
Main Results:
- Prostate cancer tissues exhibited increased expression of AMBRA1 and SQSTM1 compared to benign lesions.
- Accumulation of SQSTM1 indicated a defective autophagic process in prostate cancer cells.
- Increased AMBRA1 levels positively correlated with higher Gleason scores.
- LC3II accumulation was observed in prostate cancer but not in benign lesions.
Conclusions:
- Autophagy plays a significant role in the phenotype of prostate cancer.
- AMBRA1 accumulation is linked to prostate cancer progression and may serve as a biomarker.
- Defective autophagy is a characteristic of prostate cancer cells.
