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Related Experiment Video

Updated: Apr 1, 2026

Laser Capture Microdissection of Mammalian Tissue

Laser Capture Microdissection of Mammalian Tissue

Published on: October 1, 2007

Laser Microdissection.

Andra R Frost¹, Isam-Eldin Eltoum¹, Gene P Siegal¹

¹Department of Pathology, University of Alabama at Birmingham, Birmingham, Alabama.

Current Protocols in Molecular Biology

|October 2, 2015

Summary

This summary is machine-generated.

Laser microdissection (LM) precisely isolates cells from tissues for molecular analysis. This guide details specimen preparation, staining, and lysis buffer protocols for effective cell isolation and study.

Keywords:

cytologic samples isolated cells microdissection processing tissues

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Last Updated: Apr 1, 2026

Laser Capture Microdissection of Mammalian Tissue

Laser Capture Microdissection of Mammalian Tissue

Published on: October 1, 2007

Laser Microdissection for Species-Agnostic Single-Tissue Applications

Laser Microdissection for Species-Agnostic Single-Tissue Applications

Published on: March 31, 2022

Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion

Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion

Published on: April 12, 2020

Area of Science:

Cell Biology
Molecular Biology
Histology

Background:

Laser microdissection (LM) enables targeted cell isolation from complex tissues.
Understanding cell-specific roles in physiology and disease requires precise isolation methods.

Purpose of the Study:

To provide comprehensive protocols for preparing various specimens for laser microdissection.
To detail methods for cell visualization and lysis buffer preparation for molecular analysis.

Main Methods:

Mammalian frozen and fixed tissue preparation, including freezing, processing, embedding, and sectioning.
Cytologic specimen preparation, cell processing, and staining techniques (H&E, IHC).
Image-guided cell targeting and lysis buffer formulation for nucleic acid and protein recovery.

Main Results:

Detailed protocols cover tissue freezing, processing, embedding, sectioning, and cell preparation.
Staining methods like H&E and IHC are included for cell visualization.
Recipes for lysis buffers ensure efficient recovery of nucleic acids and proteins.

Conclusions:

Effective preparation of tissue or cytologic specimens is critical for successful laser microdissection.
The presented protocols facilitate the precise isolation of specific cell populations for downstream molecular analysis.
This unit serves as a valuable resource for researchers utilizing laser microdissection in various biological studies.