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Expression of the pneumolysin gene in Escherichia coli: rapid purification and biological properties
T J Mitchell1, J A Walker, F K Saunders
1Department of Microbiology, University of Leicester, U.K.
Biochimica Et Biophysica Acta
|January 23, 1989
Abstract:
The gene for pneumolysin, the thiol-activated toxin from Streptococcus pneumoniae, has been expressed in Escherichia coli. The recombinant protein has been purified using a rapid, high yield, purification procedure and has been shown to be identical with respect to N-terminal amino-acid sequence, specific activity, effect on human polymorphonuclear phagocytes and effect on human complement to the native toxin purified from the pneumococcus. This provides a large enough source of material to begin investigation of pneumolysin as a candidate for inclusion in a pneumococcal vaccine.