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Using SecM Arrest Sequence as a Tool to Isolate Ribosome Bound Polypeptides
Published on: June 19, 2012
Variation in the ribosome interacting loop of the Sec61α from Giardia lamblia
Abhishek Sinha1, Atrayee Ray2, Sandipan Ganguly3
1Department of Biochemistry, Bose Institute, P-1/12 CIT Road, Scheme VII M, Kolkata, 700054,, West Bengal, India. abhishek.boseinst.84@gmail.com.
Abstract:
The interaction between the ribosome and the endoplasmic reticulum-located Sec61 protein translocon is mediated through an arginine residue of Sec61α, which is conserved in all prokaryotic and eukaryotic orthologues characterized to date. Using in silico approaches we report that instead of arginine, this ribosome-interaction function is most likely discharged by a lysine residue in the protist Giardia lamblia. This functional substitution of the R with a K in GlSec61α may have taken place to accommodate a G-rich rRNA.
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