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Urinary uroplakin expression in cyclophosphamide-induced rat cystitis model.

H Park¹, Y S Kyung², G Lee³

¹Department of Urology, Dankook University College of Medicine, Cheonan, Korea.

Human & Experimental Toxicology

|October 2, 2015

Summary

Cyclophosphamide (CYP) causes urothelial injury, increasing urinary uroplakin II (UPII) and hematuria at 24 hours. Urinary UPII levels correlate with hematuria severity, indicating its role in early injury.

Keywords:

Cyclophosphamide cystitis hematuria urine uroplakin

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Establishment and Characterization of UTI and CAUTI in a Mouse Model

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Published on: June 23, 2015

Area of Science:

Urology
Biochemistry
Toxicology

Background:

Cyclophosphamide (CYP) is a chemotherapy agent known to induce urothelial injury.
Uroplakins are key structural proteins of the urothelium, but their role in acute injury is not fully understood.
Investigating biomarkers for CYP-induced cystitis is crucial for understanding and managing treatment side effects.

Purpose of the Study:

To investigate the role of urinary uroplakin II (UPII) levels in a rat model of cyclophosphamide (CYP)-induced cystitis.
To assess the dynamic changes in UPII expression and excretion following CYP administration.
To correlate urinary UPII levels with indicators of urothelial injury, such as hematuria and vesical weight.

Main Methods:

A rat model was established using CYP injection in female Sprague Dawley rats.
Control and CYP-treated rats were analyzed at 24 hours and 72 hours post-injection.
Measurements included vesical weight, hematuria severity, and UPII expression in bladder tissue and urine.

Main Results:

CYP treatment decreased vesical UPII messenger RNA at 24 hours, with recovery by 72 hours.
Urinary UPII and hematuria levels significantly increased at 24 hours post-CYP, subsiding by 72 hours.
Increased vesical weight was observed post-CYP, negatively correlated with vesical UPII levels at 24 hours.

Conclusions:

Urinary uroplakin II (UPII) is a potential early biomarker for cyclophosphamide (CYP)-induced urothelial injury.
Urinary UPII levels are associated with the severity of hematuria during the dynamic recovery phase.
Vesical UPII may act as a protective barrier against early CYP-related damage.