Related Experiment Video
Updated: Aug 2, 2026

18:10
Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency
Published on: June 16, 2011
30.2K
Safe sequencing depth to estimate the intra-host heterogeneity of viruses
Briefings in Functional Genomics
|October 3, 2015
Abstract:
Massively parallel sequencing allows efficient determination of genomic sequences and intra-host polymorphisms for viruses. However, the sequencing depth that guarantees safe interpretation of the observed data is unclear. We demonstrated that 10-fold genome coverage may allow safe genome assembly, and 1000-fold coverage is required to obtain reliable polymorphism estimation.

