Assessment of sperm motion characteristics from fertile and infertile men using a fully automated computer-assisted...

Sperm curvilinear velocity (Vc) and linearity (L) were analyzed in semen from 20 fertile men and from 53 patients with unexplained infertility by a computer-assisted semen analyzer (CASA). Because the frequency distribution of Vc from patient's spermatozoa showed a nongaussian distribution, comparison of mean values of Vc and L between men with unexplained infertility and fertile men is inappropriate. As an alternative, the authors compared the cumulative distribution of the percent of cells measured at increasing intervals of Vc and L, and observed a significant difference between patients and fertile men from the level of Vc30 through Vc70 micron/sec for curvilinear velocity and L2 through L6 for linearity (P less than 0.001). Furthermore, the authors coanalyzed these two parameters at the greatest point of difference (Vc40 and L3) and observed that their patient population can be differentiated into four subpopulations of subjects accordingly to their Vc40 and L3 values. In this study, 43 of the 53 patients had a motility disorder characterized by having a higher percent of cells being substantially slower and less directional than cells from normal men. However, in some patients, motion characteristics were indistinguishable from those in normal men.