Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Automated islet isolation from human pancreas.

C Ricordi1, P E Lacy, D W Scharp

  • 1Department of Surgery, San Raffaele Institute, Milan, Italy.

Diabetes
|January 1, 1989
PubMed
Summary

An automated method efficiently isolates human pancreatic islets with minimal trauma, high purity, and yield. This technique supports clinical trials for treating insulin-dependent diabetes mellitus.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Editorial - ERMPS Healthspan: to improve lifespan in physical and mental health.

European review for medical and pharmacological sciences·2023
Same author

Modulation of inflammation and immunity by omega-3 fatty acids: a possible role for prevention and to halt disease progression in autoimmune, viral, and age-related disorders.

European review for medical and pharmacological sciences·2023
Same author

The potential therapeutic role of vitamin D in inflammatory bowel disease.

European review for medical and pharmacological sciences·2023
Same author

The importance of vitamin D and omega-3 PUFA supplementation: a nonpharmacologic immunomodulation strategy to halt autoimmunity.

European review for medical and pharmacological sciences·2022
Same author

MESENCHYMAL STEM CELLS.

CellR4-- repair, replacement, regeneration, & reprogramming·2021
Same author

Editorial - Announcing changes in the Editorial Board.

European review for medical and pharmacological sciences·2021

Area of Science:

  • Endocrinology
  • Cell Biology
  • Surgical Innovation

Background:

  • Pancreatic islet isolation is crucial for diabetes research and transplantation.
  • Current methods often involve significant manual labor and can cause islet damage.
  • Improving islet yield and purity is essential for successful transplantation outcomes.

Purpose of the Study:

  • To develop and validate an automated method for human pancreatic islet isolation.
  • To ensure the method minimizes traumatic effects on islets.
  • To achieve high yield, purity, and functional viability of isolated islets.

Main Methods:

  • Automated islet isolation system with continuous digestion.
  • Ficoll density gradient purification.
  • Assessment of islet morphology, insulin content, and glucose responsiveness.
  • In vivo transplantation studies in diabetic mouse models.

Main Results:

  • Average yield of 2279 islets/g pancreas with 79% purity.
  • Isolated islets demonstrated normal morphology and beta-cell granulation.
  • Islets responded to glucose stimulation with a fivefold increase in insulin secretion.
  • Successful induction of normoglycemia after transplantation into diabetic mice.

Conclusions:

  • The automated method provides a reproducible and efficient means for isolating high-quality human pancreatic islets.
  • This technique meets critical requirements for minimal trauma, high yield, and purity.
  • The procedure shows significant promise for advancing islet transplantation therapies for type 1 diabetes.

Related Experiment Videos