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[Effects of different processing methods and durations of blood specimens on the level of soluble CD100 in peripheral
Zhijia Liu1, Chunmei Zhang2, Qi Li2
1Department of Urology, Chinese PLA General Hospital, Medical School of Chinese PLA, Beijing 100853; Beijing Key Laboratory of Immunology Regulatory and Organ Transplantation, Organ Transplant Institution of PLA, No. 309 Hospital of PLA, Beijing 100091, China.
Objective:
To investigate the effects of different processing methods and durations of blood specimens on the level of soluble CD100 (sCD100) in peripheral blood.
Methods:
We included a total of 15 healthy individuals without any significant signs or symptoms of microbial infection. Blood samples were collected in tubes with and without additives, as well as tubes containing citrate, EDTA and heparin, respectively, and processed for different durations of time. The serum samples were allowed to clot for 1 hour, 4, 8 hours after venipuncture before centrifugation. Then the sCD100 levels were measured with ELISA.
Results:
Serum sCD100 levels were higher than those in plasma, and no significant differences were observed among the citrate, EDTA or heparin treated plasma groups. The sera in the tubes without additives showed lower levels of sCD100 than those in the tubes with additives. The concentrations of sCD100 in sera increased with the prolonged processing time in room temperature.
Conclusion:
The measurement of sCD100 in blood samples may be interfered by the in vitro release from platelets. Thus, pre-treating methods for the collection of serum or plasma samples are critical in the assessment of sCD100 concentrations and should be carefully considered for the measurement of this important biomarker in both basic and clinical studies.
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