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Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins
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Complete Genome Sequence of Citrobacter freundii Myophage Michonne

Christopher L Bernal1, Victoria E Berkowitz1, Jesse L Cahill1

  • 1Center for Phage Technology, Texas A&M University, College Station, Texas, USA.

Genome Announcements
|October 3, 2015
PubMed

Insights

We sequenced the genome of the Citrobacter freundii myophage Michonne. This bacteriophage is a potential tool for combating antibiotic-resistant infections caused by this dangerous pathogen.

Area of Science:

  • Microbiology
  • Genomics
  • Bacteriophage research

Background:

  • Citrobacter freundii is a Gram-negative opportunistic pathogen.
  • C. freundii infections, such as neonatal meningitis, can be dangerous.
  • Antibiotic resistance in C. freundii highlights the need for alternative treatments.

Purpose of the Study:

  • To announce the complete genome sequence of the C. freundii-infecting myophage Michonne.
  • To describe the notable genomic features of myophage Michonne.
  • To explore the potential of myophage Michonne as a therapeutic agent against C. freundii.

Main Methods:

  • Whole-genome sequencing of myophage Michonne.
  • Bioinformatic analysis of the myophage genome.
  • Comparative genomics with related phages.

Main Results:

  • The complete genome of myophage Michonne was successfully sequenced.
  • Notable genomic features of Michonne were identified and described.
  • Michonne was characterized as a FelixO1-like myophage.

Conclusions:

  • The genome sequence of myophage Michonne provides a foundation for further research.
  • Myophage Michonne represents a promising candidate for phage therapy against C. freundii.
  • Understanding Michonne's genomic features can aid in developing targeted antimicrobial strategies.

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