Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

What is Genetic Engineering?

What is Genetic Engineering?

Genomics

Genomics

Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...

In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

Genome Annotation and Assembly

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

CRISPR Learns to Read the Epigenome.

The CRISPR journal·2026

Same author

Transcription factor collaboration enables precise T cell state engineering.

bioRxiv : the preprint server for biology·2026

Same author

Enhancer hubs govern chromatin topology and Th17 cell identity.

bioRxiv : the preprint server for biology·2026

Same author

Reprogramming of neuronal genome function and phenotype by astrocytes.

bioRxiv : the preprint server for biology·2026

Same author

CRISPR-Cas-based activation of PPARGC1A boosts endogenous mitochondria and enhances cardiac function after myocardial infarction.

Molecular therapy : the journal of the American Society of Gene Therapy·2026

Same author

Mismatch tolerance of a gRNA for CRISPR-based gene activation confers broad activity critical for cell reprogramming.

bioRxiv : the preprint server for biology·2026

Same journal

Complete sequencing of medaka genomes reveals the architecture of centromeric satellites, giant mobile elements, and sex chromosomes.

Genome research·2026

Same journal

Convergence and conflict among telomere specialized transposons across 60 million years of Drosophilid evolution.

Genome research·2026

Same journal

A unified analysis of cell type- and trajectory-associated pathways in single-cell data using Phoenix.

Genome research·2026

Same journal

Resf1 is required for proper placental development and configuration of trophoblast cell-specific heterochromatin.

Genome research·2026

Same journal

Telomere-driven replicative crisis is driven by large-scale changes in genomic architecture.

Genome research·2026

Same journal

Spatially informed reference-free cell-type deconvolution for spatial transcriptomics with SpatialCD.

Genome research·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Apr 1, 2026

Mouse Genome Engineering Using Designer Nucleases

Mouse Genome Engineering Using Designer Nucleases

Published on: April 2, 2014

Enabling functional genomics with genome engineering.

Isaac B Hilton¹, Charles A Gersbach²

¹Department of Biomedical Engineering, Duke University, Durham, North Carolina 27708, USA; Center for Genomic and Computational Biology, Duke University, Durham, North Carolina 27708, USA;

Genome Research

|October 3, 2015

Summary

This summary is machine-generated.

Genome engineering tools like CRISPR/Cas9 offer precise control over DNA, advancing biological understanding and therapeutics. These technologies are revolutionizing functional genomics and epigenome editing for future applications.

More Related Videos

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

Published on: November 12, 2012

Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines

Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines

Published on: June 2, 2018

Related Experiment Videos

Last Updated: Apr 1, 2026

Mouse Genome Engineering Using Designer Nucleases

Mouse Genome Engineering Using Designer Nucleases

Published on: April 2, 2014

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

Published on: November 12, 2012

Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines

Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines

Published on: June 2, 2018

Area of Science:

Molecular Biology
Genomics
Biotechnology

Background:

Genome engineering technologies enable precise control over genome sequence and regulation.
These tools are crucial for understanding fundamental biological processes and developing novel therapeutics.
Rapid advancements have ushered in a new era of functional genomics.

Purpose of the Study:

To review recent advances in widely adopted genome engineering platforms.
To discuss their applications in functional genomics and epigenome editing.
To explore current and future applications, limitations, and areas for advancement.

Main Methods:

Review of engineered zinc finger proteins.
Review of TAL effector nucleases (TALENs).
Review of the CRISPR/Cas9 system for genome editing and epigenome modification.

Main Results:

Detailed overview of genome editing nucleases (ZFNs, TALENs, CRISPR/Cas9).
Exploration of transcription factors for epigenome editing.
Discussion of emerging applications beyond simple gene editing.

Conclusions:

Genome engineering platforms significantly enhance functional genomics research.
These technologies hold immense potential for therapeutic development and biological discovery.
Continued advancements are expected to overcome current limitations and expand applications.