Isolation of Chloroplasts from Plant Protoplasts
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Updated: Apr 1, 2026

Isolation of Protoplasts from Tissues of 14-day-old Seedlings of Arabidopsis thaliana
Published on: August 17, 2009
Shiu-Cheung Lung1, Matthew D Smith2, Simon D X Chuong3
1School of Biological Sciences, University of Hong Kong, Pokfulam, Hong Kong SAR, China;
Abstract:
Chloroplasts can be isolated from higher plants directly following homogenization; however, the resulting yield, purity, and intactness are often low, necessitating a large amount of starting material. This protocol is optimized to produce a high yield of pure chloroplasts from isolated Arabidopsis protoplasts. The two-part method is a simple, scaled-down, and low-cost procedure that readily provides healthy mesophyll protoplasts, which are then ruptured to release intact chloroplasts. Chloroplasts isolated using this method are competent for use in biochemical, cellular, and molecular analyses.
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