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TissueCypher(™): A systems biology approach to anatomic pathology
Jeffrey W Prichard1, Jon M Davison2, Bruce B Campbell3
1Department of Pathology and Laboratory Medicine, Geisinger Medical Center, Danville, PA 17822, USA.
Journal of Pathology Informatics
|October 3, 2015
Summary
This study introduces TissueCypher™, a quantitative imaging method for analyzing epithelial and stromal biomarkers in Barrett's esophagus (BE). This systems biology approach aids in diagnosing high-grade dysplasia (HGD) by identifying significant differences between HGD and reactive atypia (RA) tissues.
Area of Science:
- Pathology
- Biomarker Discovery
- Systems Biology
Background:
- Histologic diagnosis faces observer variability; immunohistochemistry (IHC) is subjective and focuses on epithelial cells.
- Tumors involve complex interactions between epithelial and stromal cells, necessitating a systems biology approach.
- Current diagnostic methods require improved quantification and informatics for actionable clinical scores.
Purpose of the Study:
- Introduce TissueCypher™, a quantitative, multiplexed biomarker imaging approach using systems biology in anatomic pathology.
- Describe TissueCypher™ applications for understanding Barrett's esophagus (BE) tissue systems.
- Evaluate TissueCypher™ as an adjunctive diagnostic tool for BE.
Main Methods:
- Utilized the TissueCypher™ Image Analysis Platform to assess 14 epithelial and stromal biomarkers in BE biopsies.
- Analyzed biopsies from patients with nondysplastic BE with reactive atypia (RA) and BE with high-grade dysplasia (HGD).
- Extracted and evaluated biomarker and morphology features to compare HGD and RA cases.
Main Results:
- Multiple image analysis features from epithelial and stromal biomarkers, including immune markers and morphology, differed significantly between HGD and RA.
- Quantitative assessment revealed distinct patterns in biomarker expression and tissue morphology.
- The approach successfully differentiated between benign reactive changes and high-grade dysplasia in BE.
Conclusions:
- Assessing epithelial cell abnormalities alongside lamina propria cellular changes can aid BE diagnosis.
- TissueCypher™ offers a quantitative, systems biology-based adjunct to conventional pathology for BE assessment.
- This multiplexed imaging approach enhances diagnostic accuracy by integrating diverse tissue biomarkers.
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