![Quantitative SERS Detection of Uric Acid via Formation of Precise Plasmonic Nanojunctions within Aggregates of Gold Nanoparticles and Cucurbit[n]uril](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F61682.jpg&w=3840&q=50)
Quantitative determination of uric acid using CdTe nanoparticles as fluorescence probes
Dongri Jin1, Min-Ho Seo2, Bui The Huy3
1Key Laboratory of Nature Resource of Changbai Mountain and Functional Molecular (Yanbian University), Ministry of Education, Yanji 133002, China.
Biosensors & Bioelectronics
|October 4, 2015
Summary
This study presents a novel enzymatic optical method for detecting uric acid using fluorescent cadmium telluride (CdTe) nanoparticles. The method leverages the fluorescence quenching effect of hydrogen peroxide (H2O2) generated from uric acid enzymatic reactions for sensitive and accurate uric acid detection.
Area of Science:
- Nanomaterials Science
- Biochemical Analysis
- Analytical Chemistry
Background:
- Uric acid is a key biomarker for various metabolic disorders.
- Existing detection methods often lack sensitivity or require complex procedures.
- Fluorescent nanomaterials offer promising alternatives for sensitive biosensing applications.
Purpose of the Study:
- To develop a convenient and sensitive enzymatic optical method for uric acid detection.
- To investigate the use of ligand-capped cadmium telluride (CdTe) nanoparticles as fluorescent probes.
- To optimize the detection conditions for enhanced sensitivity and accuracy.
Main Methods:
- Enzymatic reaction of uric acid to generate hydrogen peroxide (H2O2).
- Fluorescence quenching of ligand-capped CdTe nanoparticles by H2O2.
- Investigation of different capping ligands (glutathione, 3-mercaptopropionic acid, thioglycerol) for CdTe nanoparticles.
- Optimization of reaction time, pH, incubation period, and concentrations of uricase and uric acid.
Main Results:
- Glutathione-capped CdTe nanoparticles exhibited excellent sensitivity to H2O2.
- Optimized conditions yielded a low detection limit of 0.10 µM for uric acid.
- A linear detection range of 0.22–6 µM was established for uric acid.
- The method demonstrated effective fluorescence quenching in response to uric acid concentration.
Conclusions:
- Ligand-capped CdTe nanoparticles serve as effective fluorescent probes for sensitive uric acid detection.
- The developed enzymatic optical method is convenient and suitable for uric acid analysis.
- This approach holds potential for clinical diagnostics and biochemical research.
