Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

2.5K
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
2.5K
IR and UV–Vis Spectroscopy of Carboxylic Acids01:28

IR and UV–Vis Spectroscopy of Carboxylic Acids

6.2K
In IR spectroscopy of carboxylic acids, the C=O bond shows a characteristic band between 1710 and 1760 cm⁻¹, and the O–H bond exhibits a broad band between 2500 and 3300 cm⁻¹.
However, the stretching absorptions for the C=O bond vary depending on the structure of carboxylic acids. The C=O bond of the free carboxylic acids shows a higher stretching frequency, 1760 cm−1, while H-bonded carboxylic acids (dimers) exhibit stretching absorptions at a lower frequency,...
6.2K
Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview01:13

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview

1.6K
Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
The ATR process begins by directing a beam...
1.6K
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

1.8K
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
1.8K
Spectroscopy of Carboxylic Acid Derivatives01:26

Spectroscopy of Carboxylic Acid Derivatives

3.3K
Infrared spectroscopy is primarily used to determine the types of bonds and functional groups. In carboxylic acid derivatives, a typical carbonyl bond absorption is observed around 1650–1850 cm−1. For esters, the absorption is recorded at around 1740 cm−1, while acid halides show the absorption at about 1800 cm−1. Another acid derivative, the acid anhydrides, exhibit two carbonyl absorption around 1760 cm−1 and 1820 cm−1, arising from the symmetrical and...
3.3K
UV–Vis Spectroscopy of Conjugated Systems01:32

UV–Vis Spectroscopy of Conjugated Systems

9.1K
Organic compounds with conjugated double bonds show strong absorption features in the UV–visible region of the electromagnetic spectrum attributed to π → π* electronic excitations. Generally, a UV–vis absorption spectrum is recorded as a plot of absorbance vs wavelength. The wavelength of maximum absorbance, which manifests as a peak in the absorption spectrum, is denoted as λmax.
One of the factors influencing λmax is the extent of conjugation in...
9.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Synchronous Rotation Dynamics in a Molecular Motor.

Journal of the American Chemical Society·2026
Same author

Tuning the size of quantum dots to enhance charge transfer and photocatalytic CO<sub>2</sub> reduction.

RSC advances·2026
Same author

Expanding the payload scope in antibody-drug conjugates by delivery of hydroxy-containing drugs through self-immolative phosphoramidates.

Nature communications·2026
Same author

TUB-010, a Novel Anti-CD30 Antibody-Drug Conjugate Based on Tub-Tag Technology, Widens the Therapeutic Window by Reducing Toxicity While Maintaining High Efficacy.

Molecular cancer therapeutics·2025
Same author

TUB-040, a Homogeneous and Hydrophilic NaPi2b-Targeting ADC with Stably Linked Exatecan, Exhibits Long-lasting Antitumor Activity and a Well-Tolerated Safety Profile.

Molecular cancer therapeutics·2025
Same author

Orthogonal Site-Specific Dual Bioconjugation of Aryl and Alkyl Thiols.

Journal of the American Chemical Society·2025

Related Experiment Video

Updated: Apr 1, 2026

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional &#960;-conjugate Systems
09:57

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

Published on: February 10, 2020

7.7K

β-Carotene Revisited by Transient Absorption and Stimulated Raman Spectroscopy.

Martin Quick1, Marc-André Kasper2, Celin Richter2

  • 1Department of Chemistry, Humboldt-Universität zu Berlin, Brook-Taylor-Str. 2, 12489, Berlin, Germany. quickmaq@chemie.hu-berlin.de.

Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry
|October 4, 2015
PubMed
Summary

Femto- and picosecond spectroscopy reveal molecular reorganization in beta-carotene's excited state. Changes in the C9-methyl group influence excited-state dynamics and intermolecular coupling with n-hexane solvent.

Keywords:
kineticsphotophysicstime-resolved spectroscopy

More Related Videos

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
09:57

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Published on: July 25, 2022

4.7K
Differential Imaging of Biological Structures with Doubly-resonant Coherent Anti-stokes Raman Scattering CARS
12:56

Differential Imaging of Biological Structures with Doubly-resonant Coherent Anti-stokes Raman Scattering CARS

Published on: October 17, 2010

14.1K

Related Experiment Videos

Last Updated: Apr 1, 2026

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional &#960;-conjugate Systems
09:57

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

Published on: February 10, 2020

7.7K
Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy
09:57

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Published on: July 25, 2022

4.7K
Differential Imaging of Biological Structures with Doubly-resonant Coherent Anti-stokes Raman Scattering CARS
12:56

Differential Imaging of Biological Structures with Doubly-resonant Coherent Anti-stokes Raman Scattering CARS

Published on: October 17, 2010

14.1K

Area of Science:

  • Photochemistry
  • Molecular Spectroscopy
  • Physical Chemistry

Background:

  • Beta-carotene is a vital carotenoid with complex excited-state dynamics.
  • Understanding its photophysical processes is crucial for applications in biology and materials science.
  • Femtosecond spectroscopy allows probing ultrafast molecular events.

Purpose of the Study:

  • To investigate the excited-state dynamics of beta-carotene in n-hexane using advanced spectroscopic techniques.
  • To elucidate the role of vibrational relaxation and molecular reorganization in beta-carotene's photochemistry.
  • To explore the influence of structural modifications (C9-methyl group) on excited-state lifetimes.

Main Methods:

  • Femtosecond transient absorption spectroscopy to monitor excited-state decay.
  • Stimulated Raman spectroscopy to probe vibrational modes.
  • Analysis of band integrals to separate electronic and vibrational dynamics.
  • Resonance conditions to select specific electronic states (S1, S2).

Main Results:

  • A picosecond process was observed during the decay of the S1 excited-state absorption, dependent on the C9-methyl group.
  • This process is attributed to reorganization on the S1 potential energy surface involving dihedral angles.
  • Stimulated Raman spectroscopy revealed a vibrational band at 1770 cm⁻¹ in S2, decaying within 200 fs into an S1 Raman line.
  • Low-frequency vibrational activity (<800 cm⁻¹) was observed in both S2 and S1 states.
  • Solvent spectral shifts indicated intermolecular coupling between beta-carotene and n-hexane.

Conclusions:

  • The C9-methyl group plays a critical role in beta-carotene's excited-state dynamics, influencing picosecond reorganization.
  • Vibrational modes undergo rapid evolution from the S2 to the S1 state.
  • Intermolecular interactions between beta-carotene and the n-hexane solvent are significant and affect solute dynamics.