Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Polyphosphate synthesis in yeast.

J Schuddemat1, R de Boo, C C van Leeuwen

  • 1Sylvius Laboratories, Department of Medical Biochemistry, Leiden, The Netherlands.

Biochimica Et Biophysica Acta
|February 9, 1989
PubMed
Summary

Phosphate-starved yeast cells synthesize polyphosphates, with chain length increasing over time. Glucose promotes faster synthesis than ethanol, and antimycin A affects this process differently in Saccharomyces cerevisiae and Kluyveromyces marxianus.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genetic and antigenic structural characterization for resistance of echovirus 11 to pleconaril in an immunocompromised patient.

The Journal of general virology·2014
Same author

A tool to assess knowledge, attitude and behavior of Indonesian health care workers regarding infection control.

Acta medica Indonesiana·2013
Same author

Systematic review and meta-analysis of antibiotic prophylaxis to prevent infections from chest drains in blunt and penetrating thoracic injuries.

The British journal of surgery·2011
Same author

Emergence of MRSA of unknown origin in the Netherlands.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2011
Same author

Bacterial aerosols during colonoscopy: something to be worried about?

Endoscopy·2010
Same author

Sugar Transport and Metal Binding in Yeast.

The Journal of general physiology·2009

Area of Science:

  • Microbiology
  • Biochemistry
  • Cell Biology

Background:

  • Polyphosphates are crucial energy reserves and signaling molecules in microorganisms.
  • Understanding polyphosphate synthesis regulation is key to cellular energy management.

Purpose of the Study:

  • To investigate polyphosphate synthesis in Saccharomyces cerevisiae and Kluyveromyces marxianus under phosphate starvation.
  • To compare the effects of different energy sources (glucose, ethanol) and metabolic inhibitors on polyphosphate formation.

Main Methods:

  • Incubation of yeast cells (S. cerevisiae, K. marxianus) with phosphate and energy sources (glucose or ethanol).
  • Analysis of polyphosphate chain length and synthesis rates over time.
  • Treatment with antimycin A to assess its impact on polyphosphate synthesis and orthophosphate accumulation.

Related Experiment Videos

Main Results:

  • Polyphosphate synthesis occurred in phosphate-starved yeast, with chain length increasing with incubation time.
  • Glucose supported faster polyphosphate synthesis than ethanol.
  • Antimycin A inhibited polyphosphate synthesis with ethanol in S. cerevisiae but not in K. marxianus, suggesting different energy utilization mechanisms.

Conclusions:

  • Yeast polyphosphate synthesis is a dynamic process influenced by energy source availability and cellular metabolic state.
  • Differential responses to antimycin A highlight distinct metabolic strategies between S. cerevisiae and K. marxianus for energy reserve management.