Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein-protein Interfaces02:04

Protein-protein Interfaces

15.0K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
15.0K
Protein Networks02:26

Protein Networks

4.7K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
4.7K
Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

7.2K
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
7.2K
Cooperative Allosteric Transitions01:58

Cooperative Allosteric Transitions

9.4K
Cooperative allosteric transitions can occur in multimeric proteins, where each subunit of the protein has its own ligand-binding site. When a ligand binds to any of these subunits, it triggers a conformational change that affects the binding sites in the other subunits; this can change the affinity of the other sites for their respective ligands. The ability of the protein to change the shape of its binding site is attributed to the presence of a mix of flexible and stable segments in the...
9.4K
Ligand Binding Sites02:40

Ligand Binding Sites

15.8K
Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
15.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rational design and synthesis of a heterocycle scaffold for DNMT3A inhibition.

Bioorganic chemistry·2026
Same author

Comparing Empirical and Physics-Based Models of Intermolecular Dispersion and Repulsion Energies.

Journal of chemical theory and computation·2026
Same author

Mapping the inhibition landscape of P-glycoprotein via conformational ensemble docking.

Scientific reports·2026
Same author

Leveraging fragment-based drug discovery to advance 3D scaffolds into potent ligands: application to the histamine H<sub>1</sub> receptor.

RSC medicinal chemistry·2026
Same author

A circadian checkpoint relocates neutrophils to minimize injury.

The Journal of experimental medicine·2025
Same author

Allosteric Mechanisms Triggering Substrate and Cofactor Binding in the SULT1A1 Dimer as Revealed by Molecular Dynamics Simulations.

Journal of chemical information and modeling·2025

Related Experiment Video

Updated: Apr 1, 2026

Author Spotlight: Streamlining Protein Target Prediction and Validation via Molecular Docking and CETSA
10:21

Author Spotlight: Streamlining Protein Target Prediction and Validation via Molecular Docking and CETSA

Published on: February 23, 2024

4.0K

Stabilization of protein-protein interaction complexes through small molecules.

Barbara Zarzycka1, Mélaine A Kuenemann2, Maria A Miteva2

  • 1Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM), Maastricht University, Maastricht, The Netherlands.

Drug Discovery Today
|October 6, 2015
PubMed
Summary

Small molecules that stabilize protein-protein interactions (PPIs) offer new therapeutic avenues. This study surveys PPI stabilizers, proposing a classification to aid structure-based design for drug discovery.

More Related Videos

Development of Inhibitors of Protein-protein Interactions through REPLACE: Application to the Design and Development Non-ATP Competitive CDK Inhibitors
10:33

Development of Inhibitors of Protein-protein Interactions through REPLACE: Application to the Design and Development Non-ATP Competitive CDK Inhibitors

Published on: October 26, 2015

11.9K
Isolation of Labile Multi-protein Complexes by in vivo Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography
10:50

Isolation of Labile Multi-protein Complexes by in vivo Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography

Published on: March 9, 2010

18.1K

Related Experiment Videos

Last Updated: Apr 1, 2026

Author Spotlight: Streamlining Protein Target Prediction and Validation via Molecular Docking and CETSA
10:21

Author Spotlight: Streamlining Protein Target Prediction and Validation via Molecular Docking and CETSA

Published on: February 23, 2024

4.0K
Development of Inhibitors of Protein-protein Interactions through REPLACE: Application to the Design and Development Non-ATP Competitive CDK Inhibitors
10:33

Development of Inhibitors of Protein-protein Interactions through REPLACE: Application to the Design and Development Non-ATP Competitive CDK Inhibitors

Published on: October 26, 2015

11.9K
Isolation of Labile Multi-protein Complexes by in vivo Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography
10:50

Isolation of Labile Multi-protein Complexes by in vivo Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography

Published on: March 9, 2010

18.1K

Area of Science:

  • Medicinal Chemistry
  • Structural Biology
  • Pharmacology

Background:

  • Protein-protein interactions (PPIs) are crucial in biological processes.
  • Most small molecules targeting PPIs function as inhibitors.
  • Stabilizing PPIs with small molecules presents a complementary therapeutic strategy, though stabilizers are currently scarce.

Purpose of the Study:

  • To conduct a comprehensive literature survey on small molecule-mediated PPI stabilization.
  • To propose a novel classification system for PPI stabilizers.
  • To facilitate structure-based design of novel PPI stabilizers.

Main Methods:

  • Systematic literature review of studies reporting small molecule PPI stabilizers.
  • Analysis of identified stabilizers based on their binding modes.
  • Classification of stabilizers according to the architecture of the protein-protein complex they stabilize.

Main Results:

  • Identification and compilation of existing small molecule PPI stabilizers from the literature.
  • Development of a classification framework for PPI stabilizers.
  • The proposed classification categorizes stabilizers by their interaction with the protein complex and binding mechanism.

Conclusions:

  • Small molecule PPI stabilization is an emerging field with significant therapeutic potential.
  • A structure-based classification of PPI stabilizers can guide future drug design efforts.
  • This classification aims to accelerate the development of novel pharmacological agents targeting PPIs.