Rapid low dose electron tomography using a direct electron detection camera
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Updated: Apr 1, 2026

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography
Published on: May 20, 2022
Vadim Migunov1, Henning Ryll2, Xiaodong Zhuge3
1Ernst Ruska-Centre for Microscopy and Spectroscopy with Electrons and Peter Grünberg Institute, Forschungszentrum Jülich, D-52425 Jülich, Germany.
Abstract:
We demonstrate the ability to record a tomographic tilt series containing 3487 images in only 3.5 s by using a direct electron detector in a transmission electron microscope. The electron dose is lower by at least one order of magnitude when compared with that used to record a conventional tilt series of fewer than 100 images in 15-60 minutes and the overall signal-to-noise ratio is greater than 4. Our results, which are illustrated for an inorganic nanotube, are important for ultra-low-dose electron tomography of electron-beam-sensitive specimens and real-time dynamic electron tomography of nanoscale objects with sub-ms temporal resolution.
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