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Updated: Apr 1, 2026
09:51
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
Published on: May 25, 2018
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CRISPR/Cas9 system as an innovative genetic engineering tool: Enhancements in sequence specificity and delivery
Young-Il Jo1, Bharathi Suresh2, Hyongbum Kim2
1Brandeis University, Waltham, MA, United States.
Biochimica Et Biophysica Acta
|October 6, 2015
Summary
CRISPR/Cas9 gene editing offers efficient genome modification but requires improvements in efficiency and reduced off-target effects. This review covers CRISPR/Cas9 advancements in specificity, delivery, and applications.
Area of Science:
- Molecular Biology
- Genetics
- Biotechnology
Background:
- CRISPR/Cas9 technology is a powerful genome editing tool derived from prokaryotic immune systems.
- It surpasses previous gene editing tools in efficiency, specificity, and manipulability.
- Further enhancements are needed to increase modification frequency and minimize off-target mutations.
Purpose of the Study:
- To review the developmental trajectory of CRISPR/Cas9 technology.
- To highlight advancements in sequence specificity and off-target effect reduction.
- To discuss improvements in CRISPR/Cas9 delivery systems and its applications.
Main Methods:
- Literature review of CRISPR/Cas9 development.
- Analysis of studies focusing on specificity enhancement.
- Examination of research on reducing off-target effects.
- Review of CRISPR/Cas9 delivery system innovations.
- Survey of recent laboratory and clinical applications.
Main Results:
- CRISPR/Cas9 technology has demonstrated significant efficiency and specificity in genome editing.
- Strategies to enhance sequence specificity and reduce off-target effects are actively being developed.
- Novel delivery systems are improving the accessibility and efficacy of CRISPR/Cas9.
- Successful applications are emerging in both research and clinical settings.
Conclusions:
- CRISPR/Cas9 technology continues to evolve, with ongoing efforts to optimize its efficiency and safety.
- Improvements in specificity, delivery, and reduction of off-target effects are crucial for its therapeutic potential.
- The technology shows promise for diverse applications in basic research and clinical medicine.
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