Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Water intake during drinking does not alleviate hangover symptoms.

Frontiers in pharmacology·2026

Same author

Two case reports of ovarian clear cell carcinoma with central necrosis representing a mille-feuille-like appearance on contrast-enhanced MRI.

Radiology case reports·2026

Same author

Asymptomatic and symptomatic cardiac toxicity associated with immune checkpoint inhibitors: insights from a Japanese registry.

European heart journal open·2026

Same author

Comparative Pathophysiology of Humans and Hibernating Bears: From Metabolic Failure to Adaptive Resilience.

Internal medicine (Tokyo, Japan)·2026

Same author

GRAPPA 2025 Annual Meeting: Advances in Psoriatic Disease Research From Pilot Grant Awardees.

The Journal of rheumatology·2026

Same author

A DPP inhibitor suppresses periodontitis via antibacterial effect targeting Porphyromonas gingivalis.

Scientific reports·2026

Search research articles

Related Experiment Video

Updated: Apr 1, 2026

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos

Published on: February 22, 2016

Parturition failure in mice lacking Mamld1.

Mami Miyado¹, Kenji Miyado², Momori Katsumi¹

¹Department of Molecular Endocrinology, National Research Institute of Child Health and Development, Tokyo 157-8535, Japan.

Scientific Reports

|October 6, 2015

Summary

This summary is machine-generated.

Mamld1 gene knockout in mice disrupts parturition by impairing functional luteolysis, leading to pregnancy failure. MAMLD1 regulates 20α-HSD expression, crucial for initiating labor.

More Related Videos

Generation of Maternal Mutants Using zpc:cas9 Knock-in Zebrafish

Generation of Maternal Mutants Using zpc:cas9 Knock-in Zebrafish

Published on: July 22, 2025

Author Spotlight: A Pipeline to Analyze Lineage-Specific Mutant Embryos at Single-Cell Resolution

Author Spotlight: A Pipeline to Analyze Lineage-Specific Mutant Embryos at Single-Cell Resolution

Published on: June 14, 2024

Related Experiment Videos

Last Updated: Apr 1, 2026

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos

Quantitative Analysis of Protein Expression to Study Lineage Specification in Mouse Preimplantation Embryos

Published on: February 22, 2016

Generation of Maternal Mutants Using zpc:cas9 Knock-in Zebrafish

Generation of Maternal Mutants Using zpc:cas9 Knock-in Zebrafish

Published on: July 22, 2025

Author Spotlight: A Pipeline to Analyze Lineage-Specific Mutant Embryos at Single-Cell Resolution

Author Spotlight: A Pipeline to Analyze Lineage-Specific Mutant Embryos at Single-Cell Resolution

Published on: June 14, 2024

Area of Science:

Reproductive Biology
Endocrinology
Genetics

Background:

Parturition initiation in mice involves progesterone withdrawal, driven by functional luteolysis.
Functional luteolysis is characterized by increased 20α-hydroxysteroid dehydrogenase (20α-HSD) activity, mediated by prostaglandin F2α (PGF2α) signaling.

Purpose of the Study:

To investigate the role of Mamld1 in regulating parturition and functional luteolysis.
To elucidate the molecular mechanisms by which MAMLD1 influences labor initiation.

Main Methods:

Generation and analysis of Mamld1 genetic knockout (KO) mice.
Assessment of parturition, luteolysis, and gene expression in ovaries.
In vitro studies on MAMLD1's regulation of 20α-HSD (Akr1c18) expression.

Main Results:

Mamld1 KO mice exhibited parturition failure and neonatal mortality due to defective functional luteolysis.
Progesterone receptor inhibition rescued parturition in KO mice.
MAMLD1 regulates Akr1c18 expression, while Stat5b expression is increased in KO ovaries.
Dysregulation of other genes (Prlr, Cyp19a1, Oxtr, Lgals3) observed, independent of PGF2α signaling.

Conclusions:

MAMLD1 plays a critical role in initiating labor by ensuring functional luteolysis.
MAMLD1 likely regulates luteolysis via Stat5b and other genes, independently of the PGF2α pathway.