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Temperature Determination by EPR at 275 GHz and the Detection of Temperature Jumps in Aqueous Samples
Mykhailo Azarkh1, Edgar J J Groenen1
1Huygens-Kamerlingh Onnes Laboratory, Department of Physics, Leiden University , P.O. Box 9504, 2300 RA Leiden, The Netherlands.
Abstract:
Second-moment analysis along two dimensions of continuous-wave EPR spectra of nitroxides enables EPR thermometry in a broad temperature range. Simulations show that the temperature can be derived in both the slow-motion and the fast-motion regime, which is experimentally verified at 275 GHz for H2O/glycerol (50/50% by volume) and pure water. We demonstrate that this tool allows the calibration of temperature jumps induced by infrared laser irradiation of a submicroliter sample in the single-mode cavity of a 275 GHz spectrometer, which prepares for kinetic studies of processes involving paramagnetic species.
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