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In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

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Updated: Apr 1, 2026

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

Genome Editing Using Mammalian Haploid Cells.

Takuro Horii¹, Izuho Hatada²

¹Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, 3-39-15 Showa-machi, Maebashi, Gunma 371-8512, Japan. horii@gunma-u.ac.jp.

International Journal of Molecular Sciences

|October 6, 2015

Summary

Haploid embryonic stem cells (ESCs) enable straightforward genetic manipulation in mammals. These cells significantly boost CRISPR/Cas genome editing and facilitate efficient gene knockout screening.

Keywords:

CRISPR/Cas embryonic stem cell haploid

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Area of Science:

Genetics
Stem Cell Biology
Genomics

Background:

Haploid cells are valuable for gene function studies due to single allele disruption.
Recent advancements include generating haploid embryonic stem cells (ESCs) in mice, rats, and monkeys.
Haploid ESCs offer a novel platform for mammalian genome manipulation.

Purpose of the Study:

To highlight the utility of haploid cells for genetic research.
To demonstrate the enhanced capabilities of CRISPR/Cas in haploid ESCs.
To showcase the application of haploid cell lines in genome-wide screening.

Main Methods:

Utilizing haploid embryonic stem cells (ESCs) from various mammalian species.
Applying CRISPR/Cas genome editing technology in haploid ESCs.
Employing gene knockout libraries within haploid cell lines for screening.

Main Results:

Haploid ESCs facilitate simple and efficient genetic manipulation.
CRISPR/Cas system shows enhanced potential in haploid ESCs for generating knockouts and deletions.
Genome-wide screening is more effective using haploid cell lines with knockout libraries.

Conclusions:

Haploid ESCs represent a powerful tool for advancing genetic studies and genome editing.
The combination of haploid cells and CRISPR/Cas accelerates the understanding of gene function.
Haploid cell lines are instrumental for high-throughput genetic screens.