Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The effect of centralized care on the management of postoperative fluctuations in plasma sodium concentration after pediatric suprasellar brain tumor surgery.

Pituitary·2026
Same author

A method for accuracy of placement analysis on radiolucent polyether-ether-keton facial implants: A case series.

Journal of cranio-maxillo-facial surgery : official publication of the European Association for Cranio-Maxillo-Facial Surgery·2024
Same author

Patient-tailored risk assessment of obstructive coronary artery disease using Rubidium-82 PET-based myocardial flow quantification with visual interpretation.

Journal of nuclear cardiology : official publication of the American Society of Nuclear Cardiology·2023
Same author

Implementing human factors in anaesthesia: guidance for clinicians, departments and hospitals: Guidelines from the Difficult Airway Society and the Association of Anaesthetists: Guidelines from the Difficult Airway Society and the Association of Anaesthetists.

Anaesthesia·2023
Same author

Human factors in anaesthesia: a narrative review.

Anaesthesia·2023
Same author

Machine learning based model to diagnose obstructive coronary artery disease using calcium scoring, PET imaging, and clinical data.

Journal of nuclear cardiology : official publication of the American Society of Nuclear Cardiology·2023

Related Experiment Video

Updated: Apr 1, 2026

Role of Diffusion MRI Tractography in Endoscopic Endonasal Skull Base Surgery
09:53

Role of Diffusion MRI Tractography in Endoscopic Endonasal Skull Base Surgery

Published on: July 5, 2021

4.4K

Dentatorubrothalamic tract localization with postmortem MR diffusion tractography compared to histological 3D

J Mollink1,2, K M van Baarsen3, P J W C Dederen4

  • 1Nuffield Department of Clinical Neurosciences, FMRIB Centre, University of Oxford, Oxford, UK. jeroen.mollink@ndcn.ox.ac.uk.

Brain Structure & Function
|October 7, 2015
PubMed
Summary

This study validates diffusion-weighted imaging (DWI) tractography for mapping the dentatorubrothalamic tract (DRTT). Postmortem tractography showed high accuracy when compared to 3D histology, improving reliability for neuroscience research.

Keywords:
CerebellumDentatorubrothalamic tractDiffusion-weighted imagingHistological reconstructionPostmortemTractography

More Related Videos

Co-analysis of Brain Structure and Function using fMRI and Diffusion-weighted Imaging
17:06

Co-analysis of Brain Structure and Function using fMRI and Diffusion-weighted Imaging

Published on: November 8, 2012

27.2K
Diffusion Tensor Magnetic Resonance Imaging in the Analysis of Neurodegenerative Diseases
09:33

Diffusion Tensor Magnetic Resonance Imaging in the Analysis of Neurodegenerative Diseases

Published on: July 28, 2013

29.5K

Related Experiment Videos

Last Updated: Apr 1, 2026

Role of Diffusion MRI Tractography in Endoscopic Endonasal Skull Base Surgery
09:53

Role of Diffusion MRI Tractography in Endoscopic Endonasal Skull Base Surgery

Published on: July 5, 2021

4.4K
Co-analysis of Brain Structure and Function using fMRI and Diffusion-weighted Imaging
17:06

Co-analysis of Brain Structure and Function using fMRI and Diffusion-weighted Imaging

Published on: November 8, 2012

27.2K
Diffusion Tensor Magnetic Resonance Imaging in the Analysis of Neurodegenerative Diseases
09:33

Diffusion Tensor Magnetic Resonance Imaging in the Analysis of Neurodegenerative Diseases

Published on: July 28, 2013

29.5K

Area of Science:

  • Neuroimaging
  • Neuroanatomy
  • Histology

Background:

  • Diffusion-weighted imaging (DWI) tractography estimates white matter tracts but lacks known accuracy rates.
  • The dentatorubrothalamic tract (DRTT), a key cerebellar output, is crucial for understanding cerebellar functions beyond motor control.

Purpose of the Study:

  • To compare postmortem DWI tractography of the DRTT with its 3D histological reconstruction.
  • To estimate the reliability and spatial accuracy of tractography algorithms for the DRTT.

Main Methods:

  • Postmortem MRI (7T) of a human brain specimen using DWI.
  • Probabilistic tractography (PROBTRACKX) of the DRTT.
  • Serial sectioning, myelin staining, and 3D histological reconstruction.
  • Image registration and comparison between MRI tractography and histology using ROC analysis and similarity index (SI).

Main Results:

  • High sensitivity and specificity of tractography for the DRTT were observed.
  • The superior cerebellar peduncle showed the highest spatial concordance (SI = 0.72).
  • Lower overlap was noted at the DRTT decussation in the mesencephalon.

Conclusions:

  • Postmortem DWI tractography demonstrates high spatial accuracy for the DRTT compared to 3D histology.
  • This validates tractography as a reliable tool for studying the DRTT.
  • Offers promising prospects for investigating structure-function relationships in cerebellar disorders.