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Related Experiment Video

Updated: Apr 1, 2026

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Rapid assay of stem cell functionality and potency using electric cell-substrate impedance sensing.

Michael J Rutten1, Bryan Laraway2, Cynthia R Gregory3,4,5

  • 1Center for Regenerative Medicine, Oregon Health & Science University, 3181 SW Sam Jackson Park Road, 97239, Portland, OR, USA. ruttenm@ohsu.edu.

Stem Cell Research & Therapy
|October 7, 2015
PubMed
Summary

A new assay using Electric Cell-substrate Impedance Sensing (ECIS) technology rapidly measures bone marrow mononuclear cell (BM-MNC) invasion. This advancement offers timely data for regenerative medicine and cell therapy potency testing.

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Area of Science:

  • Regenerative Medicine
  • Cell Biology
  • Biotechnology

Background:

  • Autologous bone marrow mononuclear cells (BM-MNCs) show promise in regenerative medicine, with clinical outcomes linked to their invasive capacity.
  • Current methods for assessing BM-MNC invasiveness, like the Boyden-chamber assay, are time-consuming (36 hours), labor-intensive, and provide only single time-point data, limiting clinical applicability.
  • A need exists for a rapid, sensitive, and reproducible assay to evaluate stem cell invasive function for clinical decision-making.

Purpose of the Study:

  • To develop a rapid, sensitive, and reproducible invasion assay for evaluating stem and progenitor cell invasive capacity.
  • To adapt Electric Cell-substrate Impedance Sensing (ECIS) technology for measuring BM-MNC invasion.
  • To provide timely data for patient selection in regenerative medicine and for cell product potency assays.

Main Methods:

  • Employed Electric Cell-substrate Impedance Sensing (ECIS) technology integrated with a Transwell system.
  • Utilized Matrigel-coated Transwell filters to mimic the extracellular matrix barrier.
  • Measured chemokine-directed BM-MNC cell invasion across the filter in real-time.

Main Results:

  • The developed ECIS-Transwell system enabled measurement of BM-MNC invasion within minutes.
  • Demonstrated the ability to rapidly and sensitively assess stem and progenitor cell invasive capacity.
  • Provided a platform for obtaining robust, unambiguous, and reproducible data.

Conclusions:

  • The ECIS-Transwell chamber system offers a practical solution for rapid assessment of cell invasion.
  • This assay facilitates timely evaluation of stem cell functionality for regenerative medicine applications.
  • The system has potential as a cost-effective potency assay for cell product release and regulatory approval.